Transcriptome Analysis Of The Related Genes Of Muscovy Ducks Aggressive Behavior

In order to study the cause of the attack of Muscovy Duck from the angle of molecular genetics,further improve the welfare level of Muscovy ducks and better serve the production of animal health and safety,this experiment applies the medial The Recorder2.0 software performed a 1-month(24h)behavior observation record for120 Muscovy ducks with good growth conditions,and used the instantaneous observation method and the continuous observation method to select the Muscovy Ducks(test group ?),The attacked Muscovy Ducks(test group ?)and the Muscovy Ducks(control groups)who did not show any aggressive behavior and behaved normally,and used the Observer XT behavioral software to define the behavior of the120 Muscovy ducks and to analyze the differences and links between the behaviors.In this experiment,the differentially expressed genes in the hypothalamus of three groups of Muscovy Ducks(three repetitions in each group)were screened by the Illumina HISEQ 2000 high throughput sequencing technique,using go and KEGG database to annotate the differential table genes.and to carry out the cluster analysis of differentially expressed genes.The results show:1.There was a significant positive correlation between circling behavior and pawgrasping,fighting,feathering,anal and drinking behaviors(R=0.975,P<0.05;R=0.973,P<0.05;R=0.956,P<0.05;R= 0.968,P<0.05;R=0.956,P<0.05),significantly negatively correlated with stretching behavior(R=-0.967,P<0.05);claw grasping behavior was significantly positively correlated with defecate,licking,and fighting behaviors.(R=0.995,P<0.01;R=0.998,P<0.01;R=0.994,P<0.01),showing a significant negative correlation with stretching behavior(R=-0.961,P<0.05).There was a significant negative correlation between other modification behaviors and alert behaviors(R=-0.991,P<0.01),and there was a significant negative correlation between alert behaviors and fighting,deafness,and licking behavior(R=-0.961,P<0.05;R=-0.985,P<0.05;R=-0.957,P<0.05),positively correlated with avoidance and head-rising behavior(R=0.953,P<0.05;R=0.954,P<0.05);evasive behavior and fighting and licking There was a significantly negative correlation between behaviors(R=-0.991,P<0.01;R=-0.997,P<0.01),and there was a significant negative correlation with the anal licking behavior(R=-0.951,P<0.05);fighting behavior and deafness There was a significant positive correlation between behaviors(R=0.956,P<0.05),and there was a significant positive correlation between deafness behavior and licking-anal behavior(R=0.998,P<0.01),and there was a significant positive correlation with drinking behavior(R=0.955,P <0.05);licking behavior was significantly positively correlated with drinking water and heading behavior(R=0.964,P<0.05;R=0.965,P<0.05).There was a significant positive correlation between drinking behavior and feeding behavior(R=0.990,P<0.01),and there was a significant positive correlation with swallowing(R=0.974,P<0.05).There was a very significant positive correlation between feeding behavior and swallowing behavior(R =0.992,P<0.01);Nod behavior was positively correlated with bow and mouth opening(R=0.989,P<0.05;R=0.951,P<0.05)2.Use RNA-Seq technology to screen out the SNPs,InDel sites and variable shear events generated during the biological process of attack,attack and normal behavior of all genes in Muscovy duck hypothalamus and find them above.The frequency of occurrence of genes in the hypothalamus of the Muscovy ducks was different.The occurrence of this event may lead to endocrine disorders of Muscovy ducks,as well as abnormal behaviors such as paralysis and licking.3.626 differentially expressed genes were screened out in the hypothalamus tissue of the attacking Muscovy duck,of which 137 genes were up-regulated and 489 genes were down-regulated;Differentially expressed genes were selected in the hypothalamus tissue of the Muscovy duck in the challenged group.Among them,232 were up-regulated and 417 were down-regulated.GO database analysis showed that the differentially expressed genes in the challenge group mainly involved signaling,protein binding,transport activity,and neuromodulation;the differentially expressed genes in the challenged group mainly involved signal transduction,presynaptic activation regions,and transmembrane receptor protein kinase activity.Other functions.KEGG database analysis showed that the differentially expressed genes in the challenge group were mainly enriched in signaling pathways such as metabolic pathways,FoxO signaling pathways,and MAPK signaling pathways;the differentially expressed genes in the challenge group were mainly enriched in ribosome,endocytosis,and mucin type O.-Glycan biosynthesis and ERBB signaling pathways 4 pathways.4.By comparing the results of the go and KEGG enrichment of differentially expressed genes in two groups of attacks and attacks,of all differentially expressed genes in the attack group and the attacked group,2 go terms were significantly enriched: behavioral and pain;in the metabolic pathway,the ERBB signaling pathway was significantly enriched.In this paper,the key candidate genes of 75 Muscovy ducks were screened out by integration analysis of the above common enrichment results.5.The 14 differentially expressed genes that were screened by RNA-Seq technique in relation to the aggressive behavior of Muscovy Ducks The results of the expression were consistent with the fluorescence quantitative PCR technique.