Effect Of Cadmium On Gene Expression And Function Of Poison Gland And Brain Ganglion In Pardosa Pseudoannulata

In this research,the enrichment rule of heavy metal cadmium in spider(Cd)was studied.And we revealed the effect of cadmium stress on gene expression and function of poison gland and brain ganglion of Pardosa pseudoannulata through RNA-Seq.The main results as follows:1.The fruit flies were fed with the concentration of 1.00 mg / L cadmium chloride.The concentration of cadmium in Drosophila melanogaster was peaked at 7 days.The concentration of cadmium in P.pseudoannulata increased with the time when feeding on cadmium-contained D.melanogaster and peaked at 30days(7.25?g/g),then decreased slightly and maintained a relatively stable level.Therefore,the study of enrichment of heavy metal cadmium 30 days of spiders to do transcription analysis.2.The poison ganglion were dissected from spider for RNA-Seq and 92778 Unigene with average length of 1103.9bp was obtained.Gene expression analysis showed that 237 genes were significantly different from those of normal spider glands in the spider glands(FDR?0.01,Absolute Log2foldchange> 2),up to 107,down 130.GO gene enrichment analysis,The results showed that the up-regulated genes were mainly enriched in enzyme activity(metalloproteinase activity,ubiquitin-protein transferase activity,etc.)and ion channels(calcium channel),indicating that cadmium Spore cell cycle and ion homeostasis.The down-regulated genes were mainly enriched in cholesterol-like areas,indicating that cadmium could affect the reproductive function of Pseudorabini.KEGG analysis showed that the up-regulated genes were mainly enriched in the estrogen signaling pathway and the thyroid hormone signaling pathway,indicating that cadmium could affect the reproduction and growth of the Pseudo-cyclophyta.The down-regulated genes were mainly enriched in the Rap1 signaling pathway,indicating that the cadmium Affect the immune function of the pseudo-ring.3.The ganglion were dissected from spider for RNA-Seq and 123328 Unigene with average length of 1040.73 bp was obtained.Gene expression analysis showed that 2812 genes were significantly different from those of normal spider brain ganglion(FDR ? 0.01,AbsoluteLog2foldchange> 2),which was up-regulated by 349 and down to 2463,respectively.GO gene enrichment analysis,The results showed that the up-regulated genes were mainly enriched in the synthesis and degradation of chitin,indicating that the effect of heavy metal cadmium on the immune defense function of Pardosa pseudoannulata was studied.The down-regulated genes were mainly enriched in the ion channels(sodium ion,Zinc ion),enzyme activity(protein tyrosine phosphatase,triose phosphate isomerase,ubiquitin-protein transferase activity),indicating that heavy metal cadmium affect the body of the pseudo-ring spider body stability and immune response function.KEGG analysis of differentially expressed genes was mainly enriched in oxidative phosphorylation,AMPK signaling pathway,insulin signaling pathway,thyroid hormone signaling pathway and so on,indicating that heavy metal cadmium may affect the development and individual size of Pardosa pseudoannulata spider ganglion The Predation Behavior of Panthera.4.The q-PCR was used to examine the calcium channel,RNA polymerase,integrin-mediated signal pathway-related differential gene of spider poison gland and the zygotic ion channels,tyrosine metabolic pathways,transferases,ubiquitin-protein transferase function-related genes of spider ganglion,The results were consistent with the expression of transcriptome.In conclusion,heavy metal cadmium could be transferred through the food chain and accumulated in the spider,thereby affecting the body immunity,growth,development,predation behavior and other life activities of spider.In this study,the safety of farmland spiders under cadmium stress was evaluated reasonably,which provided meaningful information for the protection and utilization of farmland spiders and the control of heavy metal pollution such as cadmium.