| The traditional experience identified Cyathula officinalis Kuan as a"good quality of less lateral roots,"that is,to assess the quality of Cyathula officinalis Kuan by the number of lateral roots..this project,the above-mentioned traditional empirical identification method is taken as the starting point.By analyzing the differences in chemical elements contained in soils from different producing areas and the endogenous phytohormone contents in Cyathula officinalis Kuan,the correlation between soil and plant hormones and the occurrence of lateral roots in Cyathula officinalis Kuan is discussed.In addition,the method of extrinsic auxin stimulation was applied to the plantlets and soil-cultured seedlings of Cyathula officinalis Kuan,and the lateral root density,primary metabolite sugar accumulation content,microscopic structure of lateral roots,and expression of related functional genes were taken as observation indexes.To investigate the effects of auxin on the lateral roots of Cyathula officinalis Kuan and its regulatory mechanisms.1.Correlation between soil information and the lateral root growth of Cyathula officinalis Kuan roots in different regionsAt present,there are 9 main producing areas in Sichuan among which there are 3production areas in Sichuan?Jinkouhe District of Leshan City,Tianquan County and Baoxing County of Ya'an City?and 6 production areas outside Sichuan?Wushan County of Chongqing,Wuxi County,Longhui County,Shaoyang City,Hunan Province,Xuanen County,Jianshi County,and Nanxun County,Hubei Province?,the number of lateral roots of Cyathula officinalis Kuan varies from region to region,including Dao's real estate district?Jinkou River,Leshan City,Sichuan Province?The roots of Achyranthes chuanxi were characterized as"long straight and few lateral roots".The number of lateral roots of Cyathula officinalis Kuan in non-Road real estate area was significantly more than that in the real estate area.The number of lateral roots of Cyathula officinalis Kuan in each main producing area was compared.Result for:Wuxi County,Chongqing>Wushan County>Hubei Province Enshi Jianshi County>Enshi Nanxun County>Hubei Province Enshi Xuanen County>Hunan Province Shaoyang Longhui County>Sichuan Province Yaan Tianquan County>Sichuan Province Bao'an County,Ya'an City>Jinkou River,Leshan City,Sichuan Province.The characteristics of chemical elements in soils from different producing areas are different,among which the chemical elements in the soils of Baoxing County in Ya'an City,Sichuan Province,Wuxi County and Wushan County in Chongqing City,Jianshi County in Enshi County in Hubei Province and Xuanen County in Enshi County in Hubei Province are similar.As a group,the soil chemical characteristics of the producing areas such as Longhui County in Hunan Province,Tianquan County in Ya'an County of Sichuan Province and Jinkou River in Leshan City are not the same as those in the above four producing areas,and the three are clustered into one.It can be seen that there is a certain correlation between the number of lateral roots of Cyathula officinalis Kuan and the content of local soil chemical elements in each producing area.However,this correlation is not close and there are some contradictions,namely the chemical element characteristics of soil in Baoxing County of Sichuan Province and Wuxi,Chongqing City.County,Wushan County,Hubei Province Enshi Jianshi County,Hubei Province Enshi Xuanen County and other production areas are similar to one another,but the number of Cyathula officinalis Kuan roots is relatively small,with Longhui County of Hunan Province and Tianquan County of Sichuan Province.Such as real estate Sichuan Achyranthes close2.Relationship between Endogenous Phytohormone Content and Number of Lateral Roots of Cyathula officinalis KuanThe ratio of endogenous hormones in plants collected from different years of the Jinkou River in Leshan City,Sichuan,and different species of Cyathula officinalis Kuan has certain differences,among which the endogenous plant hormones of Cyathula officinalis Kuan of different ages are similar to each other and are clustered together.The endogenous phytohormone contents of Cyathula officinalis Kuan and Cyathula officinalis Kuan in different age groups were similar and clustered into a large group.It can be seen that there are endogenous plant hormone levels in Cyathula officinalis Kuan and Cyathula officinalis Kuan,and Cyathula officinalis Kuan in different years.More obvious differences.From the comparison of endogenous plant hormone content,it can be found that the content of gibberellin and abscisic acid in Cyathula officinalis Kuan was higher than that of Cyathula officinalis Kuan and Cyathula officinalis Kuan in the four-year growth period,but the number of lateral roots of Cyathula officinalis Kuan was higher than that of Cyathula officinalis Kuan.Cyathula officinalis Kuan,the actual measurement results are contradictory to the reports in the literature,and the endogenous plant hormone content comparison results show that the content of auxin and cytokinin of Cyathula officinalis Kuan has a significant difference with hybrid.Cyathula officinalis Kuan and Cyathula officinalis Kuan,ie,The content of auxin in Cyathula officinalis Kuan was lower than that in Cyathula officinalis Kuan.3.Effect of Auxin/IAA on the Lateral Root Growth of Cyathula officinalis KuanThe root density of lateral roots was observed and measured after hormone treatment on the roots of the tissue culture seedlings and the leaves of the soil-cultured seedlings.The experimental results showed that under the stimulation of exogenous auxin,tissue culture and soil culture The lateral root growth of the seedlings was promoted and the lateral root density increased.With the increase of auxin concentration,the average lateral root density of tissue culture seedlings showed an upward trend,in which the maximum root density of tissue culture seedlings appeared at the concentration of auxin of 1.83×10-5mg/ml,while the concentration of auxin was applied.When it is larger than 1.83×10-3mg/ml,the morphogenesis of the tissue culture roots is affected,that is,the growth of the main root is inhibited,the whole root system is shrunken,and the main lateral roots are difficult to differentiate and appear as“cluster roots”.The method of application was stimulated by the leaves,so the root density of soil-cultured seedlings increased with increasing auxin concentration,but the increase was not as good as that of tissue culture seedlings.Under the condition of the same auxin concentration,both the tissue culture seedlings and the soil-cultured seedling roots were affected by the polysaccharide content,and the influence trends were similar.That is to say,the content of polysaccharides in tissue culture seedlings and soil culture seedling roots increased with increasing auxin concentration,but the change trend of polysaccharides in tissue culture seedlings showed a steady increase,while that of soil culture seedlings decreased first and then increased.The difference in the variation of the polysaccharide content between tissue culture seedlings and soil culture seedlings may be due to the difference in the application of exogenous auxins.The auxin directly affects the roots of the tissue culture seedlings,and the growth hormone is applied to the soil culture seedlings.In order to spray auxin on the foliage,although auxin promotes the lateral roots of both,auxin is used for direct absorption of auxin in the culture medium,and the soil-cultured seedling is absorbed through the leaves.When auxin is transported to the roots,a certain amount of auxin is lost during the period.By observing the microscopic structure of the lateral root growth of Cyathula officinalis Kuan,we found that the roots of Cyathula officinalis Kuan begin its secondary structure and early rise,after which the cells around the middle perisylvian begin to undergo splitting activities,resulting in a newly formed layer.A few loops of heterogeneous vascular bundles are generated outwards,thereby forming a certain number of multi-circular shaped vascular bundles that are arranged in a concentric manner.In the outward expansion of the lateral root primordium of Achyranthes bidentis,the density of lateral root primordium cells was larger than that of surrounding parenchyma cells,and the cell length was longitudinally elongated in the growth direction of the lateral root primordia.After auxin treatment of tissue culture seedlings and soil cultured seedlings,the expression level of related functional genes showed a decreasing trend even though the concentration of auxin was increased.The expression level of IAA14 gene in tissue culture seedlings did not show a single downward trend.,in which the expression level of IAA14 gene is increased when the auxin concentration is increased from 0 mg/mL to 1.83×10-8mg/ml and when it is increased from1.83×10-5mg/ml to 1.83×10-2mg/ml.The phenomenon of high,and in the auxin concentration of 1×10-5mg/ml,IAA14 gene expression reached the lowest minimum,soil expression of IAA14 gene expression by the application of auxin in the situation and the tissue culture seedlings are similar but The difference is that as the concentration of auxin is gradually increased,when the concentration of auxin is 0mg/ml to 1.83×10-8 mg/ml and 1.83×10-6mg/ml to 1.83×10-5mg/ml.The expression level of IAA14 gene in the seedlings did not increase,but it still showed a slowly decreasing trend.It was speculated that the method of applying auxin in soil-cultured seedlings depends on the absorption of leaves and transport in the body.This process inevitably results in more auxin.Loss and consumption,resulting in that the promotion threshold was not reached at the applied concentration of auxin of1.83×10-2mg/ml,when the auxin treatment concentration was greater than1.83×10-5mg/ml,the expression of IAA14 gene in the cultured seedlings continued to decline slowly,and the lateral root density continued to increase. |
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