Dose Effects Of Polysaccharides From The Roots Of Cyathula Officinalis Kuan On Antioxidative Activity In Mice

At present, more and more attentions have focused on the negative effects of free radicals. Excessive production and accumulation of free radicals can cause DNA damage, mutation of organisms and enven induce a variety of physiological diseases. There are some free radicals around our daily life. As an important Chinese medicines from Si chuan, Cyathula officinalis Kuan has been reported for its functions of diminishing inflammation, antitumor, improving immunity, inhibiting tumor etc. The present study was aimed to determine the effects of doses of Polysaccharides from the roots of Cyathula officinalis on antioxidant activity in mice.Initially, we have prelimilarly investigated the antioxidant capacity of Polysaccharides from the roots of C. officinalis. Tweenty-two female "Kunming mice weighing25～30g at the age of6to8weeks were were divided into two groups at random. Eleven mice received successively intragastric administration of0.25mL Polysaccharides of C. officinalis (100mg/kg/day) as long as for21days. While the other eleven mice received intragastric administration of0.25mL saline solution (0.9%) and served as normal group. All mice were sacrificed by decapitation on day22after fasting for24-h. The brain and liver tissue were colleced for determination the antioxidant activities of antioxidant enzymes. The results shown that intragastric administration of Polysaccharides from the roots of C. officinalis tended to increase the activities of total anti-oxidation competence (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and decrease the concentrations of malondialdehyde (MDA) in both brain and liver as compared with the normal group. These results suggest that intragastric administration is beneficial to scavenge reactive oxygen species and improve antioxidant capacity of mice.Secondly, we have investegated the Dose Effects of Polysaccharides from the roots of Cyathula officinalis kuan on antioxidative activity in mice. Seventy-seven mice were divided into7groups at random (n=11). Ⅰ group, mice received subscutanious daily injection of0.2mL saline solution (0.9%) and the same time intragastrically administrated with0.25mL saline solution (normal group); Ⅱ group, mice received daily subscutanious injection of0.2mL D-galactose (120mg/kg/day) and the same time intragastrically administrated with0.25mL saline solution (0.9%)(model group or negative control group); Ⅲ group, mice received daily subscutanious injection of0.2mL D-galactose (120mg/kg/day) and the same time intragastrically administrated with0.25mL Vitamin C (100mg/kg/day)(Positive control group); Ⅳ, Ⅴ, Ⅵ, Ⅶ group, mice received subscutanious injections of0.2mL saline solution (0.9%) and the same time intragastrically administrated with0.25mL Polysaccharides from the roots of C. officinalis at dosages of50mg/kg/day,100mg/kg/day,200mg/kg/day and300mg/kg/day, respectively (dosage group). All the dosage groups were lasted for21days. All mice were sacrificed by decapitation on day22after fasting for24-h. The brain and liver tissue were colleced for determination the antioxidant activities of antioxidant enzymes.The results shown that mice of the model resulted in a significant decrease in the brain and liver total anti-oxidation competence (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities, and increase of MDA concentrations as compared with the normal group. The revealed that the D-Galactose-Induced aging mouse model was established.While the supplementary administration of different dosage of polysaccharides from the roots of C. officinalis different extent increase activities of these antioxidant enzmes,T-AOC level in both brain and liver as compared with the model group. But as the dosages of polysaccharides treatment on mice increased, there had been increased promotion effects on antioxidant enzymes and T-AOC, together with the decreased concentrations on malondialdehyde (MDA). In summary, the dose effects of polysaccharides had been proved significant. These antioxidant parameters in300mg/kg/day dosage group were significantly better than the model group in brain(P<0.05).Activities of these antioxidant enzmes, T-AOC level of the supplementary administration of different dosages of polysaccharides from the roots of C. officinalis, the dose effects of polysaccharide had been proved a certain significant. These T-SOD and GSH-Px activities, together with T-AOC level in100mg/kg/day,200mg/kg/day,300mg/kg/day dosage groups were significantly better than the model group in liver (P<0.05), the activities of CAT in200mg/kg/day,300mg/kg/day dosage groups were significantly better than the model group inliver (P<0.05), the concentrations of malondialdehyde (MDA) in50mg/kg/day,100mg/kg/day,200mg/kg/day,300mg/kg/day dosage groups were significantly better than the model group in liver, but there was no dose effects (P<0.05).Taken together, these above results firstly demonstrated that polysaccharides from the roots of C. officinalis is beneficial to improve the antioxidant capacity of mice and within the dose-range studied, there was a certain significant dose effects on activities of these antioxidant enzmes, T-AOC level and300mg/kg/day dosage is optimal. These results revealed that intragastric administration of the polysaccharides is beneficial to scavenge reactive oxygen species, can relieve and block lipid peroxidation and improve the activities of antioxidant enzmes in mice, and the polysaccharides is a certain anti-aging effects and is useful for future Provide a scientific basis for the development of application.