| The sweet cherry flesh is soft,thin,and juicy,but it is harvested at a high temperature season.It is not resistant to storage,and it is easy to appear the phenomenon of dry stem,fruit softening,browning,and decay and deterioration after harvest.Therefore,it is imperative to study the suitable technology for storage and preservation of sweet cherries.Many studies have shown that enzymes play an important role in fruit senescence and browning.However,it has been reported from the molecular point of view that the relationship between PPO,POD,CAT,SOD pathways and browning in sweet cherry fruits is rarely reported.In this study,the effect of EBR treatment on quality,physiological characteristics,enzyme activity and related gene expression of sweet cherry during storage was studied.The main conclusions are as follows:1.Treat post-harvest sweet cherries with different concentrations(5?mol/L,10?mol/L)of 2,4-epibrassinolide,5?mol/L EBR can effectively reduce the sweet cherries during low temperature storage((0±0.5)?,RH:85%?90%)Occurrence of stem stalks,browning and decay of fruits,ease of color change of rind and pulp,and increase malondialdehyde(MDA)content;delay of hardness,soluble solids,vitamin C content during fruit storage Falling can better maintain the quality and flavor of the fruit.2.5?mol/L EBR treatment can effectively reduce the activities of polyphenol oxidase(PPO)peroxidase(POD)and lipoxygenase(LOX)and increase catalase(CAT)and superoxide dismutase(SOD)Activity,can effectively delay the aging of sweet cherry.3.Compared with the control,the treatment of 5?mol/L EBR for 60 days could effectively reduce the expression of PPO gene by 15.3%,and increase the expression of catalase(CAT)gene by 25.1g and superoxide dismutase(SOD)gene by 18.9%. |
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