Isolation And Identification Of Pathogen Of Orobanche Aegyptiaca Stem-rot Disease And Biological Safety

Orobanche,parasitic weed,is a serious problem faced in the process of agricultural production,crop losses due to most countries in the world.Because of Orobanche seeds produced in large quantity,and can survive in soil for a long time,once the parasite caused serious damage to the host,so at home and abroad have attached great importance to the prevention of orobanche.Xinjiang is China’s most serious harm by Orobanche area,a serious threat to the local west muskmelon production.The prevention and control of broomrape mainly rely on manual extraction and apply methods such as herbicides.But these measures have many disadvantages,such as heavy workload,difficult operation,high cost and incomplete.At present,the world increasingly keen on prevention and treatment of fungal pathogens Orobanche host specificity,which is the current research focus of the prevention and control of broomrape.This study takes Xinjiang as the research target of Orobanche isolated pathogenic bacteria,from the root rot symptoms of Orobanche,by pathogenicity test screening strong biocontrol agent,by morphological and molecular biological identification method of biocontrol bacteria,to determine the taxonomic status,and its security is evaluated.The main results are as follows:1.This study from Xinjiang,Changji,Hami,acquired broomrape root rot disease like.116 isolates were isolated by tissue isolation method,of which there were 62 Fusarium and sp.,38 Rhizoctonia and sp.,10 Pythium and sp.,and 6 other strains.The wound inoculation of Orobanche were inoculated in vitro,7 days after inoculation,62 strains of Orobanche produce pathogenicity isolates in 116,2.According to the results of pathogenicity,screened 7 strains of virulent strains,85,X7,X47,X150,69,111 and 127 as alternative biocontrol bacteria,the growth of the safety evaluation of muskmelon.The results showed that the 7 strains had different effects on the germination rate of watermelon,but the strains 85 and X7 had obvious growth promoting effects on the host growth(plant height,fresh weight and dry weight).In addition,strains 85 and X7 did not affect the rate of melon emergence,but all strains showed different inhibitory effects on the growth of melon.