| In the present China,population and grain problem is the biggest problem we are facing,the increasing population is in need of more food supplies,China's arable land is limited,How to increase grain production in large quantities has become the main goal of agricultural production,This requires the coordination of many agricultural measures,one of the most common measures is to minimize the losses caused by diseases,insects,grass,rodents and other harmful organisms.With the development of modern agriculture,excessive application of a wide range of pesticides and fertilizers has caused great damage to the environment and human health,has become an important factor affecting the agricultural economy in the world,human health and social development.More safe,fast and effective agricultural products have become the urgent need.In recent years,microbial preparations have attracted much attention because of their environmental friendliness,disease prevention and yield increase.Especially in the aspect of soil improvement,microbial pesticides play an important role.Therefore,Search for more new,safe and effective microbial pesticides and On the basis of this,establish a Benificial Microbial Motivated holistic Organic plant system,Use today's rapidly growing new media channels,such as WeChat and other public service platform,help the rapid development of organic farms to meet the growing market demand and enhance the effect of environment friendly,help learning organic growers in organic farming technology and the concept of international frontier and the establishment of cooperation platform.To help more and more Chinese people understand and accept the real,healthy and safe organic life has become a major concern.In this paper,we will evaluate the greenhouse growth promoting effects of 2 kinds of endophytic bacteria with biocontrol potential,and 38 combinations of 14 species with biocontrol potential bacteria.Tomato powdery mildew was founded to promote the whole organic cultivation system and disease control,screening of beneficial microbes driven tomato on the demonstration,2015-2016 "National Organic/green farm training and seminar" and the corresponding propaganda on the line "nnbio" WeChat public platform.1.Growth promoting evaluation of two kinds of Plant growth-promoting rhizobacteria(PGPR)and the select of tomato tomato powdery mildew biocontrol bacteriaThis experiment selected Bacillus amyloliquefaciens 54 and Bacillus subtilis JC03,which was Plant growth-promoting rhizobacteria(PGPR),compared with water treatment for greenhouse experiment on tomato.Through the detection of chlorophyll,plant height,stem diameter,leaf number,fresh and dry weight,fruit setting rate and biomass,and soluble solids,soluble sugar,soluble protein,Vc and lycopene contents in quality detection,we found that the strains of JC03,54 has a good promoting effect,54 strains of tomato growth promoting increase 22%yield.Greenhouse experiments showed that 54 and JC03 can be used as biocontrol strains for further experimental biocontrol effect,further preparation for the later research of microbial pesticides.Through this greenhouse experiment,it was proved that 54 and JC03 could be used as a new experiment for the biocontrol effect of the biocontrol strain,and the preparation of the microbial pesticide could be further prepared.We also selected the laboratory pre-screening of the 12 biocontrol strains,according to different effects,began to mix and match.Screening field tomato powdery mildew biocontrol bacteria,The purpose of this research is to study the different disease biocontrol agent combination on Tomato downy mildew and growth promoting effect.In the greenhouse experiment,the whole growth period was treated 3 times.On the twenty-fifth day,the biocontrol effect of 54+7Ze3+HS10 on Tomato powdery mildew was up to 31.67%.Secondly,the control effect of 54+JC03+7Ze3 on Tomato powdery mildew was 15.93%,and the control effect of tomato powdery mildew was in combination with 54+JC03+SM21.Although their biocontrol effect has not been more than the organic certification of microbial fertilizer products "Nan Jing shield",but the single bacteria can be used as a potential biocontrol strains for the next step of screening.2.The demonstration of Benificial Microbial Motivated holistic Organic plant system(Tomato)and the extension of China organic agriculture promotion online and offline platformWe applied Benificial Microbial Motivated holistic Organic plant system to tomato in March 2016 on Suzhou City Agricultural Biotechnology Co.Ltd.Cao Lake,Cao Hu agricultural garden.The demonstration was divided into two treatments of Benificial Microbial Motivated holistic Organic plant system and conventional cultivation system.Benificial Microbial Motivated holistic Organic plant system,using of biological pesticides and fertilizers were adopted in the whole process of Benificial Microbial Motivated holistic Organic plant system.Through soil testing indexes,promoting indexes and quality indexes,were detected two soil system after the planting.Benificial Microbial Motivated holistic Organic plant system,ammonium nitrogen,organic phosphorus and available potassium in soil than conventional planting has significant differences,and the Benificial Microbial Motivated holistic Organic plant system significantly increased the soil fertility,improve soil properties.This results indicted that Benificial Microbial Motivated holistic Organic plant system significantly increased the soil fertility.46 days after transplanting,compared with conventional cultivation system,the plant height,stem diameter,and Chlorophyll of Benificial Microbial Motivated holistic Organic plant system was increased significantly;The results show that the Benificial Microbial Motivated holistic Organic plant system has obvious growth promoting effect of tomato.At last,we future detected tomato and nutritional quality,the protein,the vitamin C content was significantly higher than that of conventional cultivation system,Titration acid content were significantly lower than that of conventional cultivation system,the soluble sugar content higher than conventional cultivation system.Meanwhile,the content of lycopene is significantly higher than conventional cultivation system.Benificial Microbial Motivated holistic Organic plant system of tomato is worth promoting,because the advantages of Benificial Microbial Motivated holistic Organic plant system can reflected from all aspects.In this study,the research team also innovatively established a "beneficial microorganism driven whole organic/green planting system",and actively promoted its development.In 2015 and 2016,successfully held the "National Organic/green planting farm training and seminar" in Nanjing Agricultural University.As an important organic event in our country,it has a high visibility and influence in Nanjing Agricultural University.The significance of the activity is to promote the development of China's organic cause,and the world,and for our school to become the world's leading Kasetsart University to make due contributions.On the content of the activities,in 2015 the organic farming farm training and seminars focused on the domestic organic plant planning,management and technical progress.The 2016 meeting on the basis of the previous year,the angle to the top international organic farming and organic agriculture development at home and abroad,organic farming enthusiasts introduced feasible low input and high output of organic farm operation mode.Activities related to publicity in the WeChat nnbio public platform cumulative harvest Wuwanwuqianyu times the amount of reading activities,communication range,effects of population wide,become a favored by the majority of enthusiasts looking forward to organic farming activities,provides a good platform for the development of organic industry Chinese. |
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