Further Study On The Rapid Propagation Technology And Leaf Regeneration System About Elite Hazelnut Resources

Hazelnut belong to Corylaceae(Corylus L.).Hazelnuts is one of the most popular nuts because of their delicious taste and rich nutrition.With the successful breeding of C.heterophylla Fisch.×C.avellana L.,the planting area of hazelnut also increased year by year,and the quality identification of cultivated varieties is also in progress.At present,several of well-behaved cultivars have been identified,such as Dawei,Liaozhen 9 and Fuzhen 4.Dawei has become the main cultivar of hazelnut cultivation in China..Seedlings are in short supply in the market.In order to improve the rapid propagation system of hazelnut tissue culture,the experiments were carried out on the five cultivars of Liaozhen 9,Liaozhen 3,Dawei,84-487 and Fuzhen 4.In addition,the regeneration system of hazelnut leaves was explored in the hope of providing references for future studies on molecular biology and breeding of hazelnut.The results are as follows:1.The best explant of hazelnut is the stem segment of indoor hydroponics.The best disinfection method is to use 0.1 % Hg Cl2 for 9 min,with a contamination rate of 15 % and a survival rate of 70 %.2.The optimal medium for Dawei primary culture is NRM+0.01 mg/L IBA+0.1 mg/L GA3+5.0 mg/L 6-BA.Fuzhen 4 and 84-487 can also be cultured in this medium.3.When the number of times of secondary culture of hazelnut was too long and the proliferation coefficient decreased,adding appropriate cytokinin TDZ could improve its proliferation.In this experiment,0.01 mg/L of TDZ was added to the original proliferation medium to improve the proliferation culture of hazelnut,and the proliferation coefficient was increased from 3.37 to 5.67.4.Rooting efficiency of 1/2NRM > 1/2MS > 1/2DKW when 1/2DKW,1/2MS and1/2NRM were used as the basic rooting medium.In addition,1/2NRM medium was significantly superior to the other two medium in rooting induction.The most suitablerooting medium for Liaozhen 9 was 1/2NRM+1 mg/L IBA+30 g/L glucose+1.3 g/L PVP+6g/L agar.5.Hydroponic hazelnut leaves were disinfected with 0.1 % Hg Cl2,and the best explant disinfection time was 4 min.The contamination rate was 7.5 % and the survival rate was 92.5 %.6.The best dark culture time for callus formation in both tissue culture leaves and hydroponic leaves was 14 d.7.Callus of leaves of Liaozhen 9 had an induction rate of 35.56 % in NRM+1.5 mg/L6-BA+0.1 mg/L IBA+30 g/L glucose+1.3 g/L PVP+6 g/L agar medium.The morphology of the induced plants was the same as that of the tissue culture seedlings cultured in stem section,and the induced buds could grow normally when transferred to the culture medium with a proliferation coefficient up to 5.17.8.In this study,the technology system of tissue culture and rapid propagation of hybrid hazel was improved,and it was proved that leaf regeneration was also one of the feasible methods of resource propagation.