Determination Of Organochlorine Pesticide Residues In Angelica

Angelica sinensis is one of the commonly used drugs in people's daily life,and is used most often in pharmaceuticals and health care products.With the improvement of people's quality of life and the development of society,people pay more and more attention to this safety hazard of Angelica pesticide residues,and people's awareness of food safety used to get stronger and stronger.Organochlorine pesticides are mostly used for crops to control pests and diseases.Because they had good effects on pest control,they will be the pesticides with the largest range of use and relatively high usage.At the same time,the use of organochlorine pesticides in a wide area and large amount of inevitability willed lead to excessive levels of organochlorine pesticide residues in Angelica.In China,due to the large amount of organochlorine pesticides,long use time,long half-life,and low degradation,it still appeared in Chinese herbal medicines.As people's awareness of food safety had gradually increased,the standards for residues of organochlorine pesticides in Chinese herbal medicines have gradually became stricter.In this paper,30 samples of Angelica sinensis from ten major Chinese angelica production bases were selected,and the optimized QuEChERS method was used to detect?-BHC,?-BHC,?-in 30 samples of Angelica sinensis by GC-ECD.Organochlorine pesticide residues such as BHC,?-BHC,PP'-DDE,OP'-DDT,PP'-DDD,PP'-DDT and PCNB were analyzed and tested,and a more convenient and efficient detection and analysis method was established.The main findings of this paper are as follows:1.Optimization of experimental methods in this experiment?1?Experiment Based on the traditional QuEChERS method,the extraction and purification of Angelica samples were optimized experimentally.After optimization,we determined the extraction of organochlorine pesticides from Angelica with formic acid-n-hexane,using a mixed reagent of 0.600 g of sodium chloride?NaCl?and 1.20 g of anhydrous magnesium sulfate?MgSO₄?as a water scavenger,then 40.0 mg.A reagent obtained by mixing octadecyl?C18?and 40.0 mg of ethylenediamine-N-propylsilane?PSA?was used as a purifier to obtain an extract of an angelica sample.?2?The experiment used the ECD detector to establish the GC-ECD technology,and used this technique as the method to obtain the chromatographic data in this experiment.The optimized method is used for the detection.The analysis results were as follows:the overall linear coefficient of the detection method was?0.995,and the LOD value calculated by the signal-to-noise ratio was between 0.300-6.00?g/L,and the LOQ value was between 0.90-18.0.Between?g/L,the RSD values were all less than 9.64%,and the spiked recovery rate of the whole experiment was between 84.5%and 118%.All the above parameters were in line with the analysis requirements of the Chinese Pharmacopoeia for various pesticide residues.2.The selected Angelica meets the Chinese Pharmacopoeia's organochlorine pesticide residue standard.Through the analysis of the obtained chromatogram and data,we measured the total residue of BHC?200?g/kg,the total residue of DDT?200?g/kg,and the total residual amount of quintonitrobenzene?100?g/kg.This experiment shows that the Angelica of the ten major Chinese herbal medicines selected in the experimental samples meet the standards for organochlorine pesticide residues in the 2015 Chinese Pharmacopoeia.Therefore,this experiment could fully prove that the Chinese herbal medicines in the ten Chinese herbal medicine production bases were safe and qualified medicinal materials that meet the standards for organochlorine pesticide residues,and all meet the safety standards for consumption,and could be safely consumed.