Effects Of Ageing On The Vigor Of Different Peanut Cultivate

Peanut is one of the important cash crops in China.Its seeds are rich in protein,fat and various micronutrients.In actual production,the ageing of peanut seed after storage leads to the decrease of seed vigor and the waste of production resources.Therefore,high-activity peanut varieties were selected to understand the physiological and biochemical characteristics of peanut ageing process.It is of great theoretical and practical significance to clarify the physiological and biochemical changes of different peanut varieties after ageing treatment.This experiment is divided into two parts.The first part to 23 peanut varieties(lines)of natural ageing seeds stored at room temperature(one year)and artificial ageing treatment(T = 40 ?,90% RH =)0,2,4,8,10 days,excavation under different ageing treatment 23 ageing resistance properties of peanut varieties(lines),selecting and resistance to ageing and ageing sensitive materials;In the second part,two selected peanut varieties were further treated with artificial ageing to study the changes of activity indexes,quality indexes,physiological and biochemical and related gene expressions of NH23 and FH18 after different artificial ageing treatments(0,2,4,6,8 and 10 days).The main test results are as follows:1.The seed vigor indexes of 23 peanut varieties(lines)after different ageing treatments were determined,and the comprehensive analysis was carried out by fuzzy mathematics membership function method,and NH23 and FH18,which were resistant to ageing and sensitive to ageing,were screened out.2.The near-infrared grain analyzer was used to determine the quality indexes of NH23 and FH18 seeds after artificial ageing.It was found that the lipid oxidation and decomposition rate of NH23 seeds was slower than that of FH18 seeds under the stress of the same ageing time.The oil content and fatty acid(linoleic acid,oleic acid)content of FH18 seeds varied more than that of NH23 seeds.3.The electrical conductivity and germination rate of NH23 and FH18 seeds were determined,and the results showed that with the extension of ageing time,the anti-ageing ability of NH23 seeds was stronger than that of FH18 seeds.At the same ageing treatment time,the conductivity of the leaching solution of FH18 seeds was significantly higher than that of NH23 seeds,while the germination rate was far lower than that of NH23 seeds.The physiological and biochemical indexes of NH23 and FH18 under different stress treatments were determined.The results showed that after a long period of ageing,the activities of SOD,POD,CAT and APX of seed hypocolexes could effectively maintain the balance of reactive oxygen species and play an important role in maintaining the higher activity of NH23 seed hypocolexes.The antioxidant stress capacity of NH23 seed was significantly higher than that of FH18 seed with the extension of ageing time.In the same ageing treatment,the increase of soluble protein in the hypocotyl of FH18 was lower than that of NH23,and the accumulation of MDA content and reactive oxygen species was higher than that of NH23.4.For validation step ageing stress related gene expression level,using ageing stress related genes of NH23 with FH18 ageing time node to analyze differentially expressed genes,the results showed that with the extension of ageing time,the NH23 and FH18 hypocotyl of Gm UBC2 gene expression were raised,under the same ageing treatment NH23 quantity is higher than Gm UBC2 gene expression in embryonic axis FH18 hypocotyl.