Effect Of Fermented Soybean Meal On Antioxidant Status,Colostrum Quality And Reproductive Performances In Sows

Our study was to study the anti-oxidative ability and oxidative damage during different physiological stages and different parities in sows,and to test the biotransformation efficacy of soybean isoflavones and the antioxidant ability of FSBM?SMF?SM in vitro.Then we evaluated the effect of FSBM on antioxidant status,colostrum quality and reproductive performances in sows.This can solve the problems of oxidative stress of sows in modern and intensive aquiculture,and provide a theoretical for improving the productivity in sows.The first part to test the anti-oxidative ability,oxidative damage of the different physiological stages(gilt,puberty,days 30,60,90 of gestation and open period)and different parities(days 1,2-3,4-5,6-8 of pregnancy)sows.The results showed that compared with the other periods' sows,the levels of SOD,ORAC,HORAC,MDA,cortisol in farrowing sows were significantly difference(P < 0.05),and the content of T-AOC and 8-ISO-PGF2? in farrowing and weaning sows were significantly increased(P < 0.05).For the parity,the levels of T-AOC and ORAC in sows from the 6th to 8th pairty were significantly decreased,the contents of MDA and 8-ISO-PGF2? in sows from the 6th to 8th pairty were significantly increased compared with the other periods' sows(P < 0.05).In conclusion,farrowing sows had higher oxidative stress and the sows from the 6th to 8th parity had lower anti-oxidative ability.The second part was aimed to study the antioxidant ability of FSBM?SMF?SM in vitro.Prepared three kinds of soybean insoflavones extract,tested the biotransformation efficacy and antioxidant ability in vitro of soybean insoflavones.The results showed that the DPPH free radical scavenging capability and reducing power increased depending on the daidzein concentration in soybean insoflavones.Hydroxyl radical and superoxide anion scavenging capability firstly increased with the sample daidzein concentration and then remained stable.Under the same concentration,the DPPH radical scavenging activity,reducing power,hydroxyl radical scavenging activity and superoxide anion scavenging activity of 3 different SM were FSBM > SMF > SM.The antioxidant capacity of FSBM was greater than the common SMF(P < 0.05),which is greater than raw SM(P < 0.05).The third part evaluated the effect of FSBM on antioxidant status,colostrum quality and reproductive performances of sows.44 sows were selected,and randomly allotted to four groups(n = 11 sows/groups).One group of sows were fed with soybean meal-based diet(control),and three groups were fed with 2%,4%,6% FSBM-based diet replacing soybean meal-based diet(2-FSBM,4-FSBM,6-FSBM),respectively.The results showed that there was no difference in the litter size,alive litter size and birth weight of piglets during the late gestation(P > 0.05).On days 14 and 21 of lactation,the piglets in FSBM groups grew faster than those in control(P < 0.05).On the farrowing day,the contents of MDA,cortisol,8-ISO-PGF2? in FSBM groups were significantly improved compared with control(P < 0.05).On day 17 of lactation,antioxidative abilities of the sows fed with 4% and 6% FSBM were significantly greater than those of sows fed with SM and 2% FSBM(P < 0.05).For the analysis of colostrum content,the protein,total slid and IgG of colostrum in the 4-FSBM group were increased significantly(P < 0.05).In conclusion,SM replaced with 4% FSBM might be more suitable for the sows to improve the antioxidant status,colostrum quality and reproductive performances.The study showed that through providing different ration FSBM for diet of reproductive sows,the level of oxidative stress in reproductive sows was decreased.This study found a new approach for the healthy growth of piglets based the balance of oxidation and anti-oxidation in sows.