Effect Of Fermented Soybean Meal On Antioxidant Status,Milk Quality In Ewes And Growth Performances Of Lambs

The study was aimed to investigate the effects of maternal gestation period,parity and fermented soybean meal?FSBM?on the redox status of ewes and growth performance in lambs during late pregnancy to early lactation,which help to decrease oxidative stress of ewes during pregnancy,and provide theory for its application in the actual production in ewes.1.The experiment was to study the effects of gestational and lactational period on the redox status in goats.2-3 parities Chong Ming white goats with similar body weight and physical condition were selected.They were non-pregnant goats,pregnant goats?30 day,60 day,120 day?,parturition day and 21^st day of lactation,each item have 5 goats,30 in total.Results show that the level of cortisol indicating stress level of ewes and free radical?H₂O₂?significantly increased from the 120 ^st day of pregnancy?P<0.05?and peaked on parturition day;T-AOC,GSH-Px and SOD increaseded and twere significantly higher in lactation period than that in pregnancy period;The content of MDA?8-ISO-PGF2?and PC peaked on parturition day.After delivery,the content of 8-OHDG significantly increased and reached highest on 21st day of lactation?P<0.05?.2.The experiment was to study the effects of different parities on the redox status in goats.We choose nulliparity goats,primiparous goats,2³ parities goats,4⁵ parities goats and 6 and above parities goats,each item have 5 goats,25 in total.Results found that parities have no significant effect on the level of cortisol?P>0.05?;The content of H₂O₂ in 0-3 parities groups is significantly lower?P<0.05?than that in the 4parity and 4 above parities group;The levels of SOD and GSH-Px were significantly decreased in the6 and 6 above parities;Also,the content of MDA,8-ISO-PGF2?,8-OHDG and PC significantly increased in 6 and 6above parities group than those in 0⁵ parities group?P<0.05?.Taken together,with the gestational period increasing,the content of free radicals increased and antioxidant capacity imcreased to to resist the free radicals.But the oxidative damage still occurred during this period.At the same time,parity decreased the antioxidant capacity and high parities goats suffered serious oxidative damage.Results implicated that we should pay attention to the oxidative status of female goats from later gestation and early lactation period and high parities goats,reinforce their nutrition control about antioxidant ability.furthermore,we study the effects of fermented soybean meal?FSBM?on oxidative status and reproductive performances during late pregnancy to early lactation of ewes.Twenty female goats at d100 of gestation were randomly divided into four groups:The control group,2%FSBM,4%FSBM,and 6%FSBM.Results showed that,compared with the control group,FSBM significantly increased?P<0.05?the litter size and the litter weight at weaning.Also,the contents of fat,protein,lactose,IgA,IgG and IgM in colostrum and milk of FSBM group are significantly higher?P<0.05?.Supplementation with FSBM increased the contents of prolactin,IGF-1 and EGF in serum especially in 4%FSBM group?P<0.05?.Compared to the control,the content of H₂O₂ in serum and placenta were significantly?P<0.05?lower,the antioxidant activities of SOD and T-AOC in serum and placenta were significantly increased?P<0.05?and the concentrations of8-ISO-PGF2?and 8-OHDG were significantly reduced?P<0.05?in 6%FESM group.In conclusion,supplememtal with FSBM during late pregnancy to lactation provided benefits on the litter size and weight of lambs,improve the milk production,increased the levels of growth hormone,immunoglobulin in milk and antioxidant enzymes,indicating that FSBM is a useful additive in ewes.Our results show thatdiet supplemented with 4%or6%FSBM is much better than 2%.