Selenium Protect Mouse Kidney From Zearalenone-induced Apoptosis Via Inhibition Of Endoplasmic Reticulum Stress-induced Apoptosis Pathways

Objective: Zearalenone(ZEA)is a non-steroidal estrogen mycotoxin widely found in animal feed and foods.It can cause diseases such as animal reproductive systems and is a major hazard to animal husbandry.Selenium is an essential trace element in the human body.In addition to participating in the normal physiological metabolism of the human body,it can effectively remove oxygen free radicals in the body,improve the body's immunity,and protect organs and tissues from oxidative damage.In addition,selenium has the functions of detoxification and detoxification on harmful substances such as aflatoxin and heavy metals.This study focused on whether selenium can protect zearalenone-induced kidney injury in mice by inhibiting endoplasmic reticulum stress(ERS)signaling pathway.Methods: Forty healthy 3 year old male Kunming mice were randomly divided into 4groups: control group(salt saline),sodium selenite(Na2Se O3)group(0.3mg/LSe),and Zea mays Enone group(70mg/kg ZEA),sodium selenite+zearalenone group(0.3mg/LSe,70mg/kg ZEA),after one week of feeding,every day after 8 hours on an empty stomach,1month after gavage,ZEA was administered.The diet was resumed after 2 hours in the stomach.After the 28 th day of gavage,after the last gavage,the rats were fasted for 8 hours,weighing the body.After anesthesia,the eyeballs were taken and blood was taken,blood was collected,kidney tissues were removed and the kidney weight was recorded.Calculate the kidney organ coefficient of each group,make renal histopathological sections,Tunel staining,detect blood biochemical indicators,tissue anti-oxidative stress index and endoplasmic reticulum stress pathway-related m RNA and protein expression,and determine Se pair ZEA causes protection of kidney damage in mice.Result:(1)ZEA caused a significant decrease in body weight,a significant decrease in renal organ coefficient,and a histological pathological damage in the kidney.Drinking drinking water containing low doses of Na2 Se O3 significantly prevented ZEA from damaging the kidneys of mice and improving their pathology.Learn to change.(2)Tunel results showed that ZEA can cause a large number of apoptosis in mouse kidney tissue cells,and drinking drinking water containing low doses of Na2 Se O3 reversed this phenomenon.(3)ZEA can significantly increase the concentration of uric acid(UA)and urea nitrogen(BUN)and creatinine(CRE)in the serum of mice,and total superoxide dismutase(T-SOD)and valley in the antioxidant enzymes of kidney tissue.The activity of glutathione peroxidase(GSH-Px)is reduced and the levels of malondialdehyde(MDA)and oxygen active substances(ROS)are significantly increased,while drinking drinking water containing low doses of Na2 Se O3 canalleviate ZEA-induced The anti-oxidation status of mouse kidney tissue decreased the content of UA,BUN and CRE in the serum of mice,decreased the content of MDA and ROS in kidney tissue,and increased the activity of T-SOD and GSH-Px.(4)Se can inhibit apoptosis and regulate the body by regulating the expression of m RNA and protein of GRP78/Bip,CHOP,JNK,P-JNK,Caspase-12,Bax and Bcl-2 genes in the endoplasmic reticulum stress pathway.Antioxidant ability to reduce the damage of ZEA to mouse kidney.Conclusion: Sodium selenite has a protective effect on oxidative damage and apoptosis induced by zearalenone in mouse kidney tissues.Sodium selenite may antagonize zearalenone by inhibiting endoplasmic reticulum stress signaling pathway.Oxidative damage in mouse kidney tissue inhibits apoptosis.