| FUT1(alpha 1-fucosyltransferase gene)and TAP1(Transporter associated with antigen processing 1)have been identified as candidate genes that offer resistance against Escherichia coli F18 infection,with the AA genotype in FUT1 and the GG genotype in TAP1 conferring resistance.In order to confirm polymorphisms at FUT1 M307 and TAP1 G729,and evaluate their influence on immunity performance in Pudong White pigs,we performed polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)analysis,measured immune indices,and compared the results with those observed in Large White pigs.The AA genotype of FUT1 was discovered in Pudong White pigs and has not been found in other Chinese domestic pig breeds.The frequency of the AA genotype in Pudong White pigs and Large White pigs was 0.018 and 0.052,respectively.The GG genotype of TAP1 was also detected in the two breeds,with a frequency of 0.708 and 0.695,respectively.Chi-square fitness analysis of both genes showed that these loci deviated from Hardy-Weinberg equilibrium in the two breeds(P < 0.05).No significant differences were observed in interleukin-6(IL-6)and IL-10 levels among the three genotypes at FUT1 and TAP1 in the two breeds(P > 0.05),implying that Pudong White pigs may have the same ability for hepatocyte inflammatory response and B cell differentiation as Large White pigs.These differences have a degree of influence on Pudong White pig's immune ability to resist F18 or other infections.TNF-? level of both disease resistance genotypes of FUT1 and TAP1 gene in the two breedswas higher than other two genotypes(P<0.05),and this indicates that the disease resistance genetypes may associated with TNF-?.The information before can facilitate us to develop subsequent studies of disease resistance and selective breeding and also to investigate the relationship between other economic characters and FUT1 TAP1 genotypes of this endemic breed. |
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