Detection Methods And Comparative Analysis Of Tetracycline In Pork And Milk

With the outbreak of more and more food safety incidents at home and abroad,the issue of food safety has been widely discussed in the international community,become the focus and focus of people's attention.It happens that the safety of animal food is particularly acute in your case.Veterinary drugs play a great role in promoting animal growth and treating animal diseases,but their toxicity is more prominent.Improper use of veterinary drugs will cause the problem of veterinary drug residues,many countries and regions as a result of veterinary drug residues caused by excessive trade disputes and food safety incidents.Tetracycline drugs are widely used in China,and their residues in animal foods have become the focus of research and detection in animal products safety inspection department.Therefore,in order to effectively monitor whether illegal drugs have been added to pork and milk,in order to detect illegal additions in time,to maintain a healthy and safe food safety environment.To understand the detection methods of tetracycline residues in animal products and to evaluate all detection methods,It is of great academic value and practical significance to understand the advantages and disadvantages of various testing methods and the suitable testing methods for county level animal product safety monitoring.For food safety monitoring and testing to lay a foundation.This paper adopts colloidal gold rapid screening technology and ELISA simple qualitative and quantitative analysis method,HPLC and HPLC-MS for the detection of tetracycline in livestock and poultry products were used to evaluate the advantages and disadvantages of the method.The main research findings are as follows:1:The content of tetracycline residues in milk samples with known additive was detected by fluorescence immunoassay and the recovery rate was between 84% and 95%.And it is convenient and quick to deal with the tedious degree of pretreatment with other methods.The sample base is large and the environmental requirements are low,but there are often false negative.Therefore,the method suitable for rapid screening and testing of primary sampling needs further confirmation for false positive false negative results.2:The recovery rate of tetracycline in pork and milk samples was determined between 83% and 101% by enzyme linked immunoassay kit.The recovery rate of milk is between 70% and 80%.The operation procedure of this method is simple,and only a simple vortex centrifuge is required,but the temperature is required to be controlled at about 25.The false positive results need to be further confirmed by HPLC or HPLC-MS.Therefore,the ELISA is the daily monitoring of the livestock product safety department of the county Bureau.3:By HPLC,the content of tetracycline residues in pork samples with known addition was determined by HPLC,The recovery rate of pork is between 97% and 118%.The positive rate of the method is low,but the effect of organic relative maps of mechanism bubbles is great.Therefore,at present,there are fewer and fewer cases of livestock product safety inspection centers in provinces and cities.4:A HPLC/MS method for the detection of four tetracycline substances in animal tissues wasestablished.The samples were extracted with 0.1mol/L Na2EDTA-Mcllvain buffer solution,filtered and centrifugal supernatant,purified with HLB solid-phase extraction column,and carbon 18 was added to acetonitrile and 0.2% formic acid water was determined by the external peak area method of liquid phase chromatography with flow phase gradient elution.This method has low false negative rate and low interference degree,which is the most suitable method for animal product safety testing center in provinces and cities.