Effect Of Matrix On Fruiting Body Character And Main Active Ingredient Content Of Cordyceps Militaris Strain

Cordyceps militaris is a type of food and medicinal fungus,with the recognition of its medicinal value,the artificial cultivation of C.militaris and the study of active substances have been concerned by many researchers.In this paper,the isolation and purification of wild strains of C.militaris,the artificial cultivation of C.militaris on rice,wheat and tussah pupae,and the cultivation of Cordyceps polysaccharides,Cordyceps acidand Cordycepin,were carried out in laboratory.The extraction and determination of four main active components of adenosine,and the effects of different culture substrates on the growth of fruiting bodies and main active components of different strains of C.militaris were compared and analyzed.The results were as follows:1.Twelve strains of C.militaris were screened from 58 wild strains.It was found that the mycelial growth rate of CM9,CM10 and CM11 was faster,there were more aerial mycelia and the edge of the colony was neat.2.Twelve strains of C.militaris were cultured on rice,wheat and tussah.Strains CM3 and CM4 grew well on rice and wheat,but generally on tussah.Strain CM6 grew poorly on rice and wheat.Strain CM9 grew well on rice,wheat and tussah,and grew best on tussah.Strain CM11grew poorly on three substrates.It can be seen that there are significant differences in fruiting body growth between different substrates and strains,and each strain has the optimum medium for growth.3.Four main bioactive components in fruiting bodies of different C.militaris strains?cordyceps polysaccharides,D-mannitol,cordycepin and adenosine?were analysed and compared on three different substrates.Fruiting body of strain CM8 on rice substrate had the highest polysaccharide content of26.73 mg.g^-1;CM11 on rice substrate had the highest content of D-mannitol and adenosine,17.21 mg.g^-1 and 2.96 mg.g^-1,respectively;Fruiting body of strain CM10 on rice substrate had the highest cordycepin content of 13.94 mg.g-1,which was higher than other strains.Fruiting body of strain CM12 on wheat substrate had the highest content of polysaccharide and D-mannitol,26.80 mg.g^-1 and 17.55 mg.g^-1,respectively;strain CM10 on wheat substrate had the highest cordycepin content of 5.92 mg.g^-1;strain CM8 on wheat substrate had the highest adenosine content of 2.41 mg.g^-1.Fruiting body of strain CM2 on Tussah pupae had the highest polysaccharide content of 25.29 mg.g^-1;strain CM12 on Tussah pupae had the highest D-mannitol content of 17.47 mg.g^-1;strain CM3 on Tussah pupae had the highest cordycepin content of 3.04 mg.g^-1;strain CM9 on Tussah pupae had the highest adenosine content of 2.62mg.g^-1.It can be seen that there are significant differences in the content of active ingredients among different strains of Cordyceps militaris.Comparing and analyzing the effect of substrate on active substances.It was found that the D-mannitol and cordycepin contents of strain CM1 on different substrates were significantly different.The polysaccharide and adenosine contents of CM8 on different substrates were significantly different.The D-mannitol and adenosine contents of CM9 on different substrates were not significantly different.The polysaccharide content of strain CM3 on different substrates were not significantly different.The cordycepin contents of fruiting bodies for strain CM7 on rice and wheat substrates were significantly higher than that on Tussah pupae.It can be seen that the substrate has a significant effect on the content of active ingredients in C.militaris.The D-mannitol contents of fruiting bodies for C.militaris purchased on the market were significantly lower than all C.militaris isolated and cultivated in our laboratory.The cordycepin contents of fruiting bodies for C.militaris purchased on the market were significantly lower than C.militaris cultivated in rice and wheat except CM7 in our laboratory.The adenosine contents of fruiting bodies for C.militaris purchased on the market were significantly lower than C.militaris cultivated in rice except CM12 in our laboratory.The polysaccharide D-mannitol and cordycepin contents of fruiting bodies for albino strain CM7were lower than most non-albino strains and the adenosine content of CM7 were higher than C.militaris cultivated in wheat substrate except CM8.