Epidemiological Investigation Of Three Kinds Of Bovine Viral Infectious Diseases In Jilin Province

To investigate the prevalence of bovine viral diarrhea(BVD),infectious bovine rhinotracheitis(IBR)and bovine respiratory syncytial(BRS)in Jilin Province,a total of 2200 serum samples(200 serum samples per counties)from cattle farms without immunization in 11 counties of 9 regions were detected by using the ELISA method and RT-PCR method.ELISA kit was used to detect the positive antibody rate of BVD,IBR and BRS of different cattle farms.The results showed that the highest IBR antibody positive rates in beef cattle farms,dairy farms,slaughterhouses,and free-range households were 31.2%,31.7%,24.5%,and 17.7%,respectively.With the analysis of the correlation between BVD,IBR and BRS antibody positive rates in each county(city),the positive rate of BVD antibody was significantly correlated with the positive rate of BRS antibody at 0.05 levels.With the systematic cluster analysis of comparing the distance between the counties(cities),there was no regularity between the difference of BVD,IBR and BRS antibody positive rates and regional differences.The mixed infection of BVD,IBR and BRS in each county(city)indicated that the positive rate of BVD antibody was significantly correlated with the positive rate of BRS antibody,the mixed infection with BVD and IBR,the mixed infection with BVD and BRS,the mixed infection with IBR and BRS,and the mixed infection with BVD,IBR,and BRS.The statistical analysis of antibody positive rate in different areas showed that the antibody positive rate of BVD,BRS were higher in the west and lower in the east,and there was a significant correlation between the two diseases in different areas.Significant difference was observed in the positive rate of antibody between the two diseases in the semi-mountain area,the plain area and the pastoral area.RT-PCR method was used to detect whether the antibody-positive serum contained viral nucleic acid or not.Results showed that the highest positive rate of dairy farms in IBR test was 26.8%;the highest positive rate of slaughterhouse in BVD test was 7.3%;the highest positive rate of dairy farms in BRS test was 8.3%.By summaring the above results,we could more specifically understood the prevalence of BIR,BVD and BRS in Jilin Province.It was preliminarily confirmed that the three diseases were common in cattle in Jilin and the infection rate of IBR was higher.The relevant prevention and control suggestions were proposed to provide data support for reducing the occurrence of the three animal epidemics,and to provide reference for the prevention and control of the three animal epidemics.