InDels Detection Of The Gene Related To Tong Sheep Tail Fat Association Analysis Between Tail Fat And Growth Trait

Tong sheep is a lipid-tailed sheep breed that is deposited by tail fat to meet its normal growth and development in harsh environments.In recent years,with the improvement of feeding conditions,the deposition of excess fat in the tail of the fat-tailed sheep has little meaning for itself,and it also causes low feed conversion rate and low meat quality.Therefore,the improvement of lean tailing of lipid-tailed sheep has become an urgent need of farmers.With the improvement of genomic research level and animal molecular breeding technology,it provides relevant theoretical basis for the improvement of fat tail and molecular breeding process.LEPR(Leptinreceptor),PPARGC1A(promoter of peroxisome proliferators-activated receptor-? coactivator 1A)of in the Adipocytokine signaling pathways and FTO(Fat mass and obesity associated gene),PDGFD(Platelet Derived Growth Factor D),PLIN1(perilipin1),UCP2(uncoupling protein 2),ETAA1(Ewing tumor-associated antigen 1)of PPAR signaling pathways and other genes have a certain correlation with sheep fat deposition,and are directly or indirectly involved in the catabolism of sheep fat.In order to verify the relationship between the polymorphism of the above genes and the deposition and growth trait of the Tong sheep tail,166 Tong sheep were studied in this study,Firstly,the above genes were subjected to InDel(Insertion/Deletion)site verification,and the corresponding primers were designed according to the verified polymorphic Loci,The gene amplification was performed by PCR amplification and agarose gel Electrophoresis,The results of statistical typing were performed,One-way analysis of variance between the tail and growth trait of the Tong sheep.aimed at studying the polymorphisms of FTO,PDGFD,UCP2,PLIN1,ETAA1,LEPR,PPARGC1 A and the Tong tail fat trait and growth trait Correlation,to provides a theoretical basis for the improvement of the Tong sheep fat tail and molecular breeding process.The research results are as follows:1.One InDel locus was detected in the LEPR gene of the Tong sheep.The waist angle width of the ewe of the InDel locus II genotype was significantly higher than that of the DD genotype(P <0.05);The body height and back height of the ram DD genotype were significantly higher compared with the genotype of II(P <0.05),sacrum high and chest depth of the ram DD genotype were significantly higher than the ID genotype(P <0.05).2.One InDel locus was verified in the in the Tong sheep PPARGC1 A gene.The body height and back height of the ewe DD genotype of this locus was significantly higher than that of the II genotype(P <0.05),and the hip high of the ram II and ID genotypes were significantly greater than the DD genotype(P <0.05).3.There was one InDel locus in the in the Tong sheep PLIN1 gene,and back high of the ewe of this locus II genotype was significantly higher than that of ID and DD genotypes(P <0.05).4.Two InDels loci were identified UCP2 gene in the Tong sheep,InDels loci of this gene had no significant effect on the tail fat and growth traits of the Tong sheep(P > 0.05).5.There was one InDel locus in the Tong sheep ETAA1 gene,and the hip width of the ram of the II genotype at the InDel locus was significantly higher than that of the DD genotype(P <0.05).6.Three InDels loci were identified in the PDGFD gene of the Tong sheep.The tail length of the ewe of the InDel-1 locus ID genotype was significantly higher than that of the II genotype(P <0.05),the InDel-3 locus DD genotype the tail length of the ewe was significantly larger than that of the ID genotype(P <0.05);The body height and body length of the ewe of the InDel-2 locus ID genotype were significantly higher than those of the DD genotype(P <0.05),and ID genotype chest depth of the ewe was significantly higher than the II and DD genotypes(P <0.05);InDel-2 locus of the ram,the body height,chest depth,back high and head length of the II and ID genotypes were significantly higher than the DD genotype(P <0.05),the ID genotype was superior to the DD genotype in the sacrum high trait of the ram(P <0.05).7.A total of 10 InDels loci were identified in the FTO gene of the Tong sheep.The tail width of the ewe DD genotype of InDel-5 locus was significantly higher than that of the ID genotype(P <0.05),and tail length of the ram ID genotype was significantly higher than DD genotype(P <0.05);InDel-4 locus tail length of the ram II and DD genotypes were significantly larger than ID genotypes(P <0.05);InDel-6 locus tail width of the ram II genotypes was significantly higher than the DD genotype(P <0.05);the InDel-9 locus ID genotype significantly increased the tail width of the ewe compared with the II genotype(P <0.05),and the DD genotype of the tail width of the ram significantly higher than ID and II genotypes(P <0.05).One-way ANOVA analysis of FTO gene polymorphism loci and Tong sheep growth trait showed that the Tong sheep FTO gene InDel-1 polymorphic locus,InDel-2 polymorphic locus,InDel-3 polymorphic locus,InDel-4 Polymorphic locus,InDel-5 polymorphic locus,InDel-6 polymorphic locus,and InDel-8 polymorphic locus had significant effects on the growth trait of the Tong sheep(P <0.05).In summary,six polymorphic loci of FTO and PDGFD genes in the Tong sheep have significant effects on the tail fat trait of the Tong sheep(P <0.05),which can be used important candidate genes as the Tong sheep fat tail modification and molecular breeding process.In addition,genetic polymorphisms such as FTO,PDGFD,PLIN1,ETAA1,LEPR and PPARGC1 A were significantly associated with the growth trait of the Tong sheep(P <0.05).