Study On Resource Evaluation Of Macrohyporia Cocos From Yunnan According To HPLC-IR-UV Data Fusion

Dried sclerotium of Macrohyporia cocos is a well-known and widely-consumed traditional Chinese medicine and is also used as dietary supplement.Both inner part(Poria)and epidermis(Poriae Cutis)of M.cocos are officially recorded in Chinese Pharmacopoeia(version 2015).Due to the difference of origin and growth pattern,the quality is uneven.Wild and cultivated M.cocos in the market are treated differently.The study on their quality comparison and provenance ability comparison were limited.Triterpenoids,one of the parameters used to evaluate the quality of tuckahoe,were usually detected by expensive and time-consuming chromatography or mass spectrometry,requiring an inexpensive and rapid alternative.In this study,high performance liquid chromatography,mid-infrared,nearinfrared and ultraviolet information were collected from 636 wild and cultivated samples of Poria and Poriae Cutis from different producing areas.Various data fusion methods including low-level,Mid-level-Boruta,Mid-level-PCA,Mid-level-VIP,High-level-PCA,High-levelBoruta,multiple information fusion of the same part and the same information fusion of two parts were investigated.Principal component analysis(PCA),partial least squares regression,partial least squares discriminant analysis and random forests were used to establish the model.The origin identification and triterpene content prediction of M.cocos were carried out,and the quality and provenance traceability of wild and cultivated products were compared.The main work of this paper was as follows:(1)Growth patterns could affect the quality of inner part and epidermis of M.cocos.Both quality and geographical traceability of cultivated and wild samples were different,and it was necessary for deliberately differential treatment.(2)Attenuated total reflectance-Fourier transform infrared(ATRFTIR)was a promising technique for the identification of wild and cultivated M.cocos.(3)The data fusion of chromatograms and spectra,and the data fusion of different parts were feasible for the accurate authentication of geographical origin.(4)Ultraviolet could provide more information about triterpene content than ATR-FTIR,and ATR-FTIR could provide more information about origin identification of M.cocos than ultraviolet.