In this study,parsley rotten root and rhizosphere soil?collectively referred to as parsley rotten root material?were used as test materials.40%alcohol was used as extractant to extract,and obtain two kinds of extract.Through column chromatography,10 fractions were collected respectively.Each fraction was mixed with PDA in the molten state.Then Fusarium oxysporium f sp.cucumerinum was inoculated it after solidification,so as to screen the best fraction for allelopathy effect.The eight best fractions collected from the first and second chromatography were detected by GC-MS to identify the main components and contents.Finally,the eight best fractions were used to study the induced resistance by the root-drenching method in the field.The research results of this study are as follows:1.Through two successive column chromatography,four fractions were collected respectively,eight of which were the best fractions.The fungal colony diameter of each fraction was significantly different from that of alcohol control?40%alcohol?and total control?sterile water?.The allelopathic effect I of RRE9 and RRE10 were 35.73%and 36.47%,and the allelopathic effect ? were 32.16%and 32.68%;the allelopathic effect I of RRE94,RRE910,RRE101 and RRE102 were 31.92%,34.37%,36.06%and 37.54%,and the allelopathic effect ? were 26.93%,29.58%,31.37%and 32.98%.The allelopathic effect I of RRSE6 and RRSE10 were 34.62%and 35.19%,and the allelopathic effect ? were 30.35%and 31.67%,the allelopathic effect of RRSE65,RRSE68,RRSE108 and RRSE109 were 45.15%,44.59%,50.32%and 50.15%,and the allelopathic effect ? were 41.51%,40.91%,47.03%and 46.85%.2.The first and second alcohol extracts of parsley rotten root materials had a significant effect on the growth and metabolism of Fusarium oxysporum.The activity of cell wall degrading enzyme?including cellulase,?-glucosidase and pectinase?was significantly different from that of control;the content of fusaric acid was significantly different from that of control;?3?the content of MDA and soluble protein was significantly different from that of control;?5?the activity of protective enzyme system?including SOD,cat,pod and PPO?was significantly different from that of control.3.GC-MS analysis was carried out on the eight best fractions of the second alcohol extracts of parsley rotten root materials,and the following allelochemials were detected respectively:Alkanes,phenols,esters and ethers are the main allelochemials in the seco-nd alcohol extracts of parsley rotten root,including 3,3-dimethylhexane?24.60%?,2-bromo nony lhydride?8.22%?,2,4-dimethylheptane?33.5%?,3,5-di-tert-buty lphenol?61.2%?,2,4-di-tert-butylbenzoic acid-5-hydroxy-n-amyl ester?17.4%?,3-meth yl-3-et-hyl-1,2-cyclopropane diamine?1.22%?,ursolic acid?1.78%?,N-4-ethoxyphe nyl-ace-tamide)?28.6%?,2-hydroxy-2-methyl-succinic acid?20.2%?,2,4,7-trishydro xy-1-napht-halenone?18.6%?,methoxyphenamine?13.5%?,Benzoyl metronidazole?9.55%?,M-flu-orophenylalanine?8.2%?,ethaneperoxoic acid,1-cyano-1-[2-?2-phe nyl-1,3-dioxolan-2-yl?ethyl]pentyl ester?2.75%?,diheptyl phthalate?1.58%?,?2E?-3-?4-methylphenyl?-1-p-henyl-2-propene-1-one?1.02%?,2-ethylhexyl vinyl ether?5.79%?,1,2-dipentyldithiane-1,1,2-trioxides?2.8%?,Allyl octyl ether?1.39%?,Glutaric acid?2,4-di-tert-butylph-enyl?monoester?8.05%?,N-[4-?diethylamino?phenyl]ac etamide?1.48%?,Alpha asp-artate phenylalanine?40,7%?,Decanedioicacid,1-methyl ester?11.5%?.The best fractions of the second alcohol extracts of parsley rotten root rhi-zosphere soil are mainly acids,esters and ketones,among which 3,5-di-tert-but y-lphenol?27.8%?and 2,4-di-tert-butylphenol?23.5%?also exist.It also include-s:methyl benzoate?22.33%?,7-chloro-2,3-dihydro-3-hydroxy-5-phenyl-lh-1,4-be nzo-diazepine-2-one?0.19%?,3,4,5-trihydroxy?propyl benzoate 0.83%?,2,4-dime thox-ybenzoic acid?5.63%?,2,Ethyl 4-dimethylbenzoate?23.38%?,allylnonyl o xalat-e?2.96%?,4-methyl-1-undene?0.73%?,decylamine?0.64%?,p-tert-butylphe nylgl-ycidyl ether?0.48%?,5-methyl-3,3-diphenyl-3h-pyrazole?0.23%?,9-ethylant hrac-ene?0.06%?,2,2-trifluoroacetamide?0.4%?,2,5-dimethoxycinnamic acid?21.81%?,n-??TERT butoxycarbonyl?-L-glutamic acid?1.81%?,3-[2-[4-?6-fluoro-1,2-benzoxazol-3,yl?-1-piperidinyl]ethyl]ethyl]-6,7,8,9-tetrahydro-2-methyl4 h-pyridin[1,2-?]pyrimidine-4-ketone?1.03%?,5-o-coffee acyl quinic acid?0.36%?,6-fluoro-3-trifluo-trifluomethyl benzoic acid-2-acyl-4,6-dichlorophenyl ester?0.05%?,4-dihydroxybenzoic acid?35.58%?,spirulinic acid?7.94%?,trimethylph osphoryl acetate?4.38%?,1'-?4-chlorophenylsulfonyl?-[1,4']pipe-ridinyl-4 '-f ormamide?1.08%?and diethyl malonate?26.63%?.The allelochemicals detected from parsley rotten root materials are mainly esters and acids.4.RRE94,RRE910,RRE101,RRE102,RRSE65,RRSE68,RRSE108 and RRSE1098 of second alcohol extracts of parsley rotten root materials were used to induce cucumber seedlings.The disease index and the effect of induced resistance were as follows:on the 9th day,the disease index of RRE94,RRE910,RRE101,RRE102,RRSE65,RRSE68,RRSE108 and RRSE1098 were 32.65%,32.25%,31.49%,30.89%,30.46%,29.62%,30.16%and 28.76%respectively;the induced resistance effect I were 55.09%,55.56%,56.64%,57.62%,58.11%,59.42%,58.48 and 60.42%;the induced resistance effect ? were 45.18%,45.77%,47.08%,48.21%,48.42%48.86%,50.39%,49.3%and 51.69%. |