Study On Liquid Spawn Cultivation And Formula Of Culture Substrate Of Sparassis Crispa

Sparassis crispa is a rare edible and medicinal fungus,which contains rich protein,vitamins and other nutrients.Its main active substance is the polysaccharide of Sparassis crispa,especially the content of beta-glucan is the highest.The polysaccharide of Sparassis crispa has the functions of immune regulation,anti-tumor,anti-inflammatory,anti-virus,anti-oxidation,anti-radiation and so on.It has become a research hotspot in recent years.In order to improve the production of Sparassis crispa and reduce the cost,this paper studies liquid strain culture,excipient selection,formula optimization and additives in order to provide technical reference for industrial production.The main results are as follows:1.The optimum carbon source,nitrogen source,metal ion and plant growth regulator for liquid culture of Sparassis crispa were screened,and the suiTable le nutrients were determined.2.The general rotary combination design was used to optimize the production formula of liquid strain of Sparassis crisp.The suiTable le formula for liquid strain culture was determined,namely yeast extract powder 0.344%,peptone 0.309%,glucose 2.05%,MgSO₄0.1%,KH₂PO₄0.2%,pH4-5.3.The technology of producing liquid strains of Sparassis crispa in shaking flask was studied by using general rotary combination design.The optimum conditions for liquid strain cultivation were determined as follows:temperature 24-26℃,pH 4-5,shaking speed170-180 r/min,and the optimum liquid content 111 mL/flask（250 mL）.4.The effects of ferrous sulfate and zinc sulfate on hyphal biomass of Sparassis crispa in liquid shake flask culture were studied.The results showed that appropriate amount of ferrous sulfate and zinc sulfate could promote the accumulation of hyphal biomass of Sparassis crispa.5.The effects of four plant growth regulators on liquid culture were studied.The results showed that appropriate amount of Kinetin and 6-BA could promote the accumulation of mycelial biomass in liquid shake flask culture of Sparassis crispa;2,4-D had no significant effect;indole acetic acid had inhibitory effect.6.The effects of corn flour,wheat bran,corn cob and soybean meal on the cultivation of Sparassis crispa.were studied.The results showed that the yield of Sparassis crispa.could be significantly increased by adding corn flour and wheat flour.Soybean meal and bran had no significant effect on the yield of Sparassis crispa.Corn cob was not conducive to the production of Sparassis crispa.7.The formula was optimized by general rotary combination design.The results showed that the optimum ratio of corn flour,wheat flour and bran could significantly increase mycelial growth rate and fruiting body yield.8.The effects of five additives on the growth and development of Sparassis crispa were studied.The results showed that three of them could promote the mycelial growth rate of Sparassis crispa.