Effects Of Glutamic Acid Residue On Rumen Effective Degradation Rate And Small Intestine Digestibility Of Common Feed For Beef Cattle

The purpose of this experiment was to study the effects of adding different levels of glutamate residues to diets on rumen fermentation indicators,blood biochemical indicators,effective rumen degradation rate,and small intestine digestibility of Qinchuan beef cattle.As solid particles of concentrated molasses fermentation broth(CMS),glutamic acid residue is rich in bacterial protein and humic acid.Rich in protein,amino acids,vitamins and other nutrients,it has the potential to serve as a protein feed for livestock and poultry.The application provides the basis.The experiment used 5 Qinchuan beef cattle with permanent rumen fistula and 3 duodenal fistulas as experimental animals.The animals were divided into 4 periods of 30 days each(including 15 days of prefeeding period and positive trial period).15 days),each period was fed a diet with different levels of glutamate residue addition(ie the first period(0%),the second period(1.5%),the third period(3.0%),the fourth period(4.5%)).In each period of experiment,nylon bag method was used to determine 18 common feeds for beef cattle(alfalfa hay,wheat straw,corn straw,corn silage,straw,straw,corn flour,tabletted corn,cooked corn,wheat bran,white spirits,beer Different time periods of dried matter(DM),crude protein(CP),neutral detergent fiber(NDF),acid detergent fiber(ADF)(6,12,24,36,48h)changes in rumen degradation rate;the small intestine digestibility of dry matter(DM)and crude protein(CP)of the 18 feeds was measured using a moving nylon bag method.Rumen fluid was collected to determine its p H,ammonia nitrogen and microbial protein content,and blood samples were collected for determination of blood biochemical indicators.The research achieved the following results:1.Addition of glutamic acid residues can improve the degradation of alfalfa hay,corn silage,straw,straw,white wine lees,beer lees,vinegar lees and rapeseed meal DM(P <0.05);Degradation of wheat,wheat straw,corn silage,straw,fungus bran,tabletted corn,cooked corn,wheat bran,vinegar residue,soybean meal,cotton meal(P <0.05);help to improve wheat straw,corn straw,corn Degradation of silage,straw,cooked corn,wheat bran,pomace,vinegar residue,rapeseed meal NDF(P <0.05);help to improve alfalfa hay,wheat straw,corn stalk,corn silage,straw,pressed corn,Degradation of wheat bran ADF(P <0.05);the addition of 3.0% glutamic acid residue was the best.2.There was no significant difference in the degradation rate of DM in the small intestine of the 18 common feed groups(P> 0.05),and the degradation rate of the DM in thesmall intestine of the 18 common feeds was less than that of the CP.The small intestine digestibility of the compressed corn CP test group ? was significantly higher than that of the control group(P <0.05).The digestive rate of small intestine in group ? and ? of cooking corn CP test was significantly higher than that in control group(P <0.05).The small intestine digestibility of wheat bran CP test group ?,?,and ? was significantly higher than that of the control group(P <0.05).The small intestine digestibility of the soybean meal CP test group? was significantly higher than that of the control group(P <0.05).The small intestine digestibility of rapeseed meal CP test group ? was significantly higher than that of control group,test groups ? and ?(P <0.05).3.With the increase of the replacement amount of glutamic acid residue,the p H of bovine rumen fluid increased,but the p H difference between the groups was not significant(P> 0.05).The concentration of ammonia nitrogen in the rumen fluid decreased,and the control group was significantly higher than that in the test groups ? and ?(P <0.05).The microbial protein content increased,and the test group ? and ? were significantly higher than the control group and the test group ?,and the test group ? was significantly higher than the test group ?.The content of propionic acid in test groups ? and ? was significantly higher than the control group(P <0.05),and the test group ? was significantly higher than the test group ?(P <0.05).The ratio of acetic acid to propionic acid in the test group II was significantly lower than the other three groups(P <0.05),and the test group III was significantly lower than the control group(P <0.05).4.The content of alkaline phosphatase in the blood of beef cattle in test group ? was significantly higher than those in the other three groups.The content of alkaline phosphatase in blood of beef cattle in control group and test group ? was significantly higher than that in test group ?(P <0.05),while the control group 3.There was no significant difference between the groups in the test ? group(P> 0.05);there was no significant difference in other blood biochemical indexes between the groups(P> 0.05).In summary,the addition of glutamate residues to replace part of the soybean meal in the diet can improve the effective rumen degradation rate of various commonly used feeds;it can improve the CP small intestine digestibility of various refined feeds.Can significantly reduce the concentration of ammonia nitrogen in the rumen,significantly increase the content of microbial protein,increase the content of propionic acid,reduce the ratio of acetic acid to propionic acid,and improve the environment in the rumen;can enhance the activity of alkaline phosphatase in the plasma of Qinchuan beef cattle and improve Qinchuan Body health of cattle.The substitution of glutamic acid residue for soybean meal can significantly improve the digestion of Qinchuan beef cattle feed,and at the same time significantly reduce the cost of feed.From this,it can be concluded that within 4.5% substitution,glutamic acid residue can replace soybean meal as protein feed The best replacement is 3%.