The Composition Of Intestinal Microbita And Immune System In AIDS Patients On Long-term Highly Active Antiretroviral Therapy

The infection of human immunodeficiency virus(HIV)as the causative organism of acquired immunodeficiency syndrome(AIDS).There is no way to completely cure AIDS,although highly active ant:iretroviral therapy(HAART)can effectively inhibit HIV replication.Intestinal including lager number of commensal gut microbe plays an important role to maintain human health,which is the major organ of early HIV replication and damage CD4+T lymphocytes.HIV the immune system releasing pro-inflammatory cytokines,damaging intestinal mucosal barrier,causing alterations in the gut microbiota and bacteria translocation,which promoting AIDS disease process.Therefore,studies of gut microbial alteration in AIDS patients and the correlation between intestinal microbiota and systemic inflammation cytokine will be better evaluate the effect in improving the prognosis of AIDS patients.Using 16S ribosomal DNA sequencing and bio informat:ion analysis methods systemic researched the structure of intestinal microbiota in health:individuals and HIV infected undergoing highly active antiretroviral therapy(HAART).At the phylum level,there are Firmicutes increased and Bacteroidetes decreased on AIDS patients who was treated with HAART.We found the ratio Firmicutes and Bacteroidetes was significant increased compared with health individuals.Alpha and'beta diversity analysis revealed that the microbiota diversity of AIDS patients undergoing HAART significant reduced compared to health individuals.At the Species level,AIDS patients undergoing HAART contain a large number of opportunistic pathogen or pathogens,such as Prevotella copri,Megaoonas funiformis,Fusobacterium mortiferum and Blautia luti,but the probiotics enriched in health individuals,including Oscillibacter sp.,Bifidobacteria,Coprococcus sp.,and so on.However,we confirmed that significant differences in bacteria translocation is real facts on HAART treated AIDS patients by using Specific primers amplification method,which including Prevotella copri and Megamonas funiformis.We measured the systemic inflammation cytokine and microbial translocation marker by using Enzyme immunoassay kit.The results of correlation between the systemic inflammation cytokine,For example,Interleukin-6(IL-6),Interleukin-10(IL-10),Tumor necrosis factor-?(TNF-?)and microbial translocation marker,such as Lipopolysaccharides(LPS),Lipopolysaccharides-binding protein(LPB),Anti-endotoxin core antibodies(EndoCAb),Soluble CD14(sCD14),revealed that systemic inflammation is positive correlated with microbial translocation.However,intestinal fatty acid binding protein(i-FABP)is non-significance correlation with IL-6 and TNF-?,but IL-10 and i-FABP is positive related.By evaluating the correlation between systemic immune cytokine and gut microbiota on Genus level,we found proinflammation cytokine and microbial translocation marker is positive relate to Erythrobacter,Candidatus phytoplasma.The research revealed that alterations in the gut microbiota associated with HAART treated AIDS patients.Pathogen Megamonas funifornis,Prevotella copri and Fusobacterium mortiferum were increased on HAART treated AIDS patients,which caused microbial translocation.In addition,the alterations of gut microbiota associated with HAART treated AIDS patients were promoted the inflammation diseases and mirobial translocatiot,which increased AIDS morbidity and mortality.