Effects Of Mifepristone On Ectopic Endometrial Growth And COX-2 And VEGF Expression In Rats

BackgroundIn recent years,with the development of economy and society in our country,the rhythm of people's life is quickening,the working pressure is increasing,and the incidence of endometriosis is increasing day by day.The pain and infertility caused by it have caused very serious physical and mental distress to women,and have also brought a certain financial burden to their families.The treatment of endometriosis has gone through a very slow development process since it was defined.However,the pathogenesis of endometriosis has not been fully elucidated,so,Endometriosis is still one of the hotspots in obstetrics and gynecology.ObjectiveTo investigate the effect of mifepristone on ectopic endometrium in rats,and to explore its pathogenesis and optimal dosage,so as to provide a new idea and method for clinical treatment of endometriosis.MethodsIn this experiment,72 model rats were randomly divided into three groups: group A: contrast group;group B: low dose group;group C: medium dose group;group D: high dose group,18 rats in each group.Routine feeding.Rats were given Mifepristone once a day.Then the rats in group A were given only peanut oil,Group B was given 10 mg/d for clinical use.Group D: 12.5mg/d,corresponding to25 mg/d.according to the experimental zoology conversion formula: dosage of rats =(dosage/60 kg)x6.25 rat body weight (kg).The dosage of mifepristone powder was 1.041 mg/mL in group B and 1.240 mg/mL ingroup C respectively.The dosage of mifepristone powder added to peanut oil to form 1 mg/mL suspension was 1 mg/mL.all the rats were killed after 4 weeks of gastric perfusion,and the ectopic tissue of the abdominal wall of the rats was taken out.The mRNA expression of COX-2mRNA ? VEGFmRNA was detected by reverse transcriptase polymerase chain reaction(RT-PCR).The expression of COX-2?VEGF was detected by Western blot.Results1.The expression of COX-2mRNA?VEGFmRNA and protein in the ectopic lesion was significantly higher than that before the model making in the ectopic lesion(P < 0.05).2.After different doses of mifepristone were given intragastric perfusion,the volume of ectopic focus was significantly reduced,the middle and high dose groups were significantly lower than those of the low dose group,and the high dose group was more obvious,the difference was statistically significant(P < 0.05).3.The correlation analysis showed that the expression of COX-2 and VEGF was significantly correlated(r =0.782,P=0.023).4.PCR technique showed that the expression of COX-2 mRNA-VEGF mRNA in the control group was higher than that in the control group,and the expression of VEGF mRNA in the low dose group,the middle dose group and the high dose group was decreased in turn(P < 0.05).5.Western blot technique showed that the expression of COX-2 protein in the control group was higher than that in the control group.The expression of COX-2 protein in the low dose group,middle dose group and high dose group decreased in turn(P <0.05).These results further explained the results of RT-PCR detection from the protein level.ConclusionMifepristone down-regulates the expression of COX-2 and VEGF.which may be the mechanism of the treatment of endometriosis.The effect of high dose mifepristone treatment is more remarkable.