Clinical Study Of VEGF And Sflt-1 In Serum Of Patients With Endometriosis

Objective:To detect the changes of VEGF and sflt-1 in Serum of Patients with Endometriosis. To be proven their action on Endometriosis by further experiments and approach the diagnostic value of sflt-1, so that we can provide a theoretical basis for early serological diagnosis on Endometriosis, at the same time, we can also provide a new idea for clinicians to treat endometriosis by antiangiogenesis.Methods:Levels of VEGF and sflt-1 in Serum were detected by ELISA in the patients with endometriosis and those without it, furthermore, the VEGF activity indices and ROC Area Under Corve of sflt-1 were calculated. At the same time, patients with endometriosis were divided two groups(group 1 including with patients who were stage I and II and group 2 including with those who were stage III and IV)and four groups, which are no pain group, mild, moderate and severe pain groups, according to the revised American Fertility Society classification (r-AFS) and the degree of severity in the dysmenorrhea respectively. Meanwhile, the relativity was analyzed between them. By using SPSS 16.0 statistical package to finish analysis of data. Results:1,In EMS group, the concentrations of VEGF and sflt-1 and VEGF activity indices in serum were elevated significantly compared with those in the control group (P<0.01); 2,In EMS group, the concentrations of VEGF in proliferative phase were elevated significantly compared with those in secretive phase (P<0.05), but no significant differences were found in the concentrations of sflt-1 and VEGF activity indices (P>0.05), in control group, no significant differences were found in the concentrations of VEGF and sflt-1 and VEGF activity indices (P> 0.05); 3,In EMS group 1, the concentrations of sflt-1 in the serum were elevated significantly compared with those of EMS group 2 (P<0.01), on the contrary, the concentrations of VEGF and VEGF activity indices were lowered significantly (P<0.01); 4,In EMS group, the concentrations of VEGF and VEGF activity indices in the serum in severe pain group were elevated significantly compared with moderate, mild and no pain group (P <0.01), the concentrations of VEGF were different in pairwise(P<0.05), otherwise, there were no significant difference between mild and no pain groups about the VEGF activity indices (P>0.008). The concentrations of sflt-1 in the serum in severe pain group were elevated significantly compared with moderate, mild and no pain group (P<0.01), at the same time, they were different in pairwise (P<0.05); 5,In EMS group, the concentrations of VEGF and VEGF activity indices in the serum correlated positively with r-AFS (P<0.01), but a negative correlation was found between the concentrations of sflt-1 and r-AFS (P<0.01); 6,In EMS group, the concentrations of VEGF and VEGF activity indices in the serum correlated positively with the degree of severity in the dysmenorrhea (P<0.01), but a negative correlation was found between the concentrations of sflt-1 and the degree of severity in the dysmenorrhea (P<0.01); 7,The ROC Area Under Corve of sflt-1 was 91.2%.Conclusions:1,VEGF and sflt-1 were closely related to the progenesis of EMS; 2,In EMS group, menstrual flow had an effect on the concentrations of VEGF in the serum, but there were not influence in the concentrations of sflt-land VEGF activity indices; 3,In EMS group, the concentrations of VEGF and sflt-1 and VEGF activity indices in the serum were all related to the r-AFS and the degree of severity in the dysmenorrhea; 4,We can have a thorough knowledge of the action of VEGF on EMS by VEGF activity index; 5,Sflt-1 had some value to the early serological diagnosis of EMS.