The Differential Expression Of SLC33A1 Gene In Peripheral Blood May Serve As A Genetic Marker For The Diagnosis Of Coronary Heart Disease

ObjectiveAccording to a previous study of the gene chip of the research group,the expression of SLC33A1 gene in the peripheral blood of patients with coronary heart disease was lower than that in the Non-CHD group.In this study,the method of expanding the clinical sample size was used to further verify the differential expression of the SLC33A1 gene in peripheral blood of more samples.To explore whether the change in the expression level of SLC33A1 gene in the peripheral blood may be used as a genetic reference for coronary heart disease risk assessment.MethodsAccording to the enrollment criteria,98 patients with coronary heart disease were selected as the experimental group,and 98 Non-CHD people were the control group.The clinical baseline data of two groups of subjects were collected in detail.Real-time fluorescence quantitative PCR was used to quantify the expression of SLC33A1 mRNA in the peripheral blood of both groups.And using clinical epidemiological methods for analysis.According to the results of percutaneous coronary angiography,the degree of coronary artery lesion in all the subjects was scored according to the gensini scoring system to further analyze the correlation between the relative expression level of SLC33A1 gene and gensini score.ResultsThe clinical baseline data of coronary heart disease group and Non-CHD group showed that there was no significant difference in age,gender,BMI,total cholesterol,low density lipoprotein cholesterol,history of hypertension,smoking history,drinking history and so on.However,there were significant differences in fasting blood glucose,diabetes mellitus,triglyceride,and high-density lipoprotein cholesterol levels.The results were statistically significant.Real-time fluorescence quantitative PCR results showed that the relative expression of SLC33A1 gene in peripheral blood of patients with coronary heart disease was lower than that in the normal control group.There was a statistically significant difference between the two group（P=0.000）.The relative expression level of SLC33A1 mRNA in the coronary heart disease group was 0.57 times that of the control group.Multivariate binary logistic regression analysis showed that the decrease of relative expression of SLC33A1 gene was an independent risk factor for coronary heart disease.The low expression of SLC33A1gene increased the risk of coronary heart disease by 5.125 times.The ROC curve was plotted based on the relative expression level of SLC33A1 gene.The results showed that the area under the curve（AUC）was 0.736±0.036,the cutoff value was 2^-△Ct=0.002883 The sensitivity of diagnosis of coronary heart disease was 0.796,the specificity was0.633,the positive predictive value was 75.61%,and the negative predictive value was 68.42%.A bivariate correlation analysis of gensini scores and relative expression levels of SLC33A1 gene in all subjects showed a negative correlation between the two（P=0.016,rs=-0.254）.Namely,the lower the relative expression level of SLC33A1 gene,the higher the gensini score,the more serious coronary stenosis.Conclusions1.Compared with the control group,the SLC33A1 gene in the peripheral blood of coronary heart disease group showed low expression at the RNA level.2.Low expression of SLC33A1 gene RNA in peripheral blood is an independent risk factor for the occurrence of coronary heart disease,which increases the risk of coronary heart disease by 5.125 times.3.The relative expression level of SLC33A1 RNA in peripheral blood may be used as a genetic marker for the diagnosis of coronary heart disease.4.The relative expression level of SLC33A1 RNA in peripheral blood may be used as a reference index for assessing coronary artery disease in patients with coronary heart disease.