Water Extraction And Enrichment Process For Total Flavonoids From Fruits Of Rosa Xanthina Lindle

Objective:The rosa xanthina lindle is a kind of rich,green,sustainable development and utilization and characteristic resources in Shanxi Province.To obtain further information for industrial production,taking efficiency into account,an economical extraction process was designed.the optimal enrichment process of total flavonoids in fruit of Rosa xanthina was discussed,providing reference for further development and utilization.Method:A single factor array design and an L₉?3³?orthogonal array design were employed to optimize the extraction condition.The effects of soak period,solid-to-liquid ratio,extraction time,extracting time first,pH of liquid and temperature were studied on extraction rate of total flavonoids,as determined by ultraviolet?UV?spectrometry.AB-8and D101 macroporous adsorbents were screened from macroporous adsorption resin,to further investigate its adsorption and desorption properties of total flavonoids in Rosa.The static test and dynamic test method were employed to obtain the optimum enrichment process.Results:The optimal extraction conditions were its p H of 3 as solvent solid-to-liquid ratio of1:10,soak period of 1.5h and 80?for an extraction duration of 1.5h.Under the optimal extraction conditions,the maximum extraction rate was up to 9.39 mg/g.The optimal enrichment conditions were the solid-to-liquid ratio of 1:10,the flow rate of 2 m L/min,the maximum sample volume of 20 BV,the eluent volume fraction of 70%,the elution flow rate of 2 mL/min,and the elution dosage of 3 BV.Conclusion:The optimal water extraction and enrichment process for total flavonoids from fruits of Rosa Xanthina Lindle obtained in this study has excellent stability.It can be a reference for industrialization.