Purification Of Flavone From Bamboo Leaves And Study On The Process Of Improving Fla Vonoe Solubility And Antioxidant A Ctivity

established by UV spectrophotometer.Comparison of ethanol,ethyl acetate,and both mixed in different proportions,n-butyl alcohol impact on the purity and yield of total flavonoids.Optimizing the extraction conditions,for the preparation of oil-soluble materials embedded products.The optimal conditions for purification of EOB were set up,with the yield and content of total flavonoids as indicators.The optimum parameters were acquired as follows:bamboo leaf flavone 1.0g,the proportion of the extraction solvent of ethyl acetate and ethanol 4:1,liquid ratio of 30:1,the extraction temperature is 60 C,extraction time 20min,extracted three times.The results showed that the yield and content of bamboo leaf flavone were 81.7%and 51.3%.Secondly,the method of solvent-melting was established to prepare oil-soluble embedding objects of bamboo leaf flavone.Established a method for detecting oil-soluble bamboo leaf flavone clarity in soybean oil to light transmittance and dissolved flavonoid oil solubility for evaluation.Through single facor experiment,screening out the optimal preparation conditions of oil-soluble flavonoids bamboo.The optimal preparation conditions of oil-soluble flavonoids was obtained as follows:bamboo leaf flavone 1.0 g,40 mL 50%ethanol,the carrier material(glyceryl monostearate)is 6.0 g,the HLB of emulsifier is 5,emulsifier dosage was 0.4%(weight compared with flavone),emulsification temperature and time was 60? 20 min,respectively.Concentration established by UV spectrophotometer.Comparison of ethanol,ethyl acetate,and both mixed in different proportions,n-butyl alcohol impact on the purity and yield of total flavonoids.Optimizing the extraction conditions,for the preparation of oil-soluble materials embedded products.The optimal conditions for purification of EOB were set up,with the yield and content of total flavonoids as indicators.The optimum parameters were acquired as follows:bamboo leaf flavone 1.0g?the proportion of the extraction solvent of ethyl acetate and ethanol 4:1,liquid ratio of 30:1,the extraction temperature is 70?,extraction time 20min,extracted three times.The results showed that the yield and content of bamboo leaf flavone were 81.7%and 51.3%.Secondly,the method of solvent-melting was established to prepare oil-soluble embedding objects of bamboo leaf flavone.Established a method for detecting oil-soluble bamboo leaf flavone clarity in soybean oil to light transmittance and dissolved flavonoid oil solubility for evaluation.Through single facor experiment,screening out the optimal preparation conditions of oil-soluble flavonoids bamboo.The optimal preparation conditions of oil-soluble flavonoids was obtained as follows:bamboo leaf flavone 1.0 g,40 mL 50%ethanol,the carrier material(glyceryl monostearate)is 6.0 g,the HLB of emulsifier is 5,emulsifier dosage was 0.4%(weight compared with flavone),emulsification temperature and time was 60?,20 min,respectively.Concentration flavonoid preparation embedded in the oil is 0.02%,the transmittance was 86.3%,the oil solution clarified,preferably oil-soluble.And lodimetry compared with different concentrations of flavonoids oil solution and flavonoids raw antioxidant effect in soybean oil.Accelerated oxidation in a thermostat(60±2)? for 8 days,the peroxide value of the product is lower than the embedding material flavonoids,indicating soluble soluble embedding material can improve the problem of poor flavonoids,but also improve the anti-oxidation effect.The result showed when concentration of flavonoids in the oil was 0.02%,the peroxide value of the measured was the least and acquired the best antioxidant effect.When the concentration of flavonoids in the oil is 0.03%,the small part is not completely soluble flavonoids affect its antioxidant effect.Finally,prepared by spray drying method of water-soluble microcapsules of bamboo leaf fiavone,and Phenanthroline Fe2+was used to determine its anti-oxidative stability in aqueous solution.Use inclusion rate of total flavonoids as indicators in order to establish the optimal conditions for preparing microcapsules.The Optimal preparation conditions of water-soluble flavone microcapsules was obtained as follows:flavone powder(35%)1.0 g was dissolved in 20 mL 50%ethanol,the wall material is ?-cyclodextrin 6.0 g,with distilled water to a concentration of 4%aqueous solution,tween 80 in an amount of 0.4%of the amount of flavones,at 60? water bath and stirred 50 min,inlet air temperature of 150?.The experimental results showed the average particle diameter of microcapsules was(152.5±10.0)nm,particle size distribution(PDI)is 0.37,the inclusion rate was 87.4%.Comparison of scavenging reactive oxygen radicals from the ability,we can know that their anti-free radical activity and Vitamin C fairly and significantly stronger than the Vitamin E,reaced 50%.After EOB microencapsulated significantly improved oxidation stability and covered up the smell of bamboo.