| Nocadia rubra cell wall skeleton,referred to as N-CWS,is an effective immune adjuvant has been used in clinical by peptidoglycan,arabinogalactan and several lipid components of a composite prodrug.At present,many studies mostly focused on the cell wall skeleton composite level research and few studies focused on the monomer components,this paper is mainly focused on the complex drug peptidoglycan monomer(N-CWS-PG)for related research.This article based on the Nocardia cells subjected to ultrasonic crushing treatment to obtain cell wall.After organic solvent extraction pigment and conducted some related researchs,including the sulfuric acid chromogenic reaction and thin-layer chromatography.The results of experiment showed the extract pigment contain pigments such as carotenoid in species.Enzyme solution was carried out on the cell wall,organic solvent treatment,drying process to obtain the cell skeleton.The cell walls skeleton was conducted by organic solvent,acid solution vacuum drying and so on,finally get pale yellow substance(shallow).The pale yellow substance was treated by lysozyme,lysozyme enzyme solution preliminary experimental results confirmed that the extraction material of peptidoglycan;Experimental extract by amino acid composition analysis showed that the structure of the amino acid composition is consistent with peptidoglycan,the main amino acid(lysine,glutamic acid,alanine)molar percentage than for 1:11.06:18.58,confirmed the extract is the peptidoglycan.In order to understand the purity of N-CWS-PG.For the peptidoglycan monomer of the protein content,total sugar content,aminocaproic sugar content,total lipid content were determined experimentally measured,the results showed that N-CWS-PG is 1.86%total lipid,protein 51.62%,9.87%neutral sugars,amino hexose 33.85%composition,the total amount of substances accounted for 97.20 percent sample of N-CWS-PG,and the results showed that the purity of the experiment comparing the extracted peptidoglycan high.Injection of peptidoglycan monomer in mice serum and use the CCK-8 detection serology experiments in vitro tumor cell staining reagents,the results showed that the drug serum on adherent growth of HepG2 cells and suspended growth of S180 cells proliferation in mice have certain inhibition.By building BALA/C mouse S180 tumor to study the inhibition of N-CWS-PG on tumor growth,measured by the spleen index,thymus index,macrophage function to study the effect of peptidoglycan on immune organs.The results showed that,N-CWS-PG on S180 solid tumor growth inhibition with a certain and the highest inhibition rate of 61.35%,the mouse spleen index and thymus index were increased and macrophage phagocytosis also have a certain upgrade..By building ascites tumor in mice and peptidoglycan gavage treatment observation peptidoglycan on ascites tumor survival of mice survival time of impact,the results showed that peptidoglycan can improve the living conditions of ascites tumors in mice and to a certain extent can prolong the survival time.In summary,the cell walls of Nocardia contains carotenoid pigment,the peptidoglycan by chemical assay of the pure display,N-CWS-PG played a certain role in inhibiting tumor growth.N-CWS-PG has a certain improvement in the quality of life of ascites tumor bearing mice and it also has a certain effect on the survival time. |
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