Study On The Active Ingredients Of Hypoglycemic And The Fingerprint Of The Leaves Of Dimocarpus Longan Lour.

Objective:1 To investigate the chemical constituents of petroleum spirit fraction and ethyl scetate fraction of the leaves of Dimocarpus longan Lour.2 Study on ?-glucosidase Inhibitory Activity of the leaves of Dimocarpus longan Lour.3 The simultaneous determination contentsfrom the leaves of the Dimocarpus longan Lour.from different habitats by HPLC.4 To establish an HPLC fingerprint in ethyl acetate fraction of the leaf of Dimocarpus longan Lour.collected from different habitats.Methods: 1 Separation and purification of the constituents ofpetroleum spirit fraction and ethyl scetate fraction of the leaves of Dimocarpus longan Lour.whichwere isolated and purified by silica gel column chromatography,also were recrystallized by different solvents.Their structures were identified by physiochemical property and spectral data analysis.2 To study the ?-glucosidase activity inhibitory between the diferent polarity components and the different chemical components in the leaf of Dimocarpus longan Lour.by 96 microwell plate.3 The simultaneous determination contents,which were ethyl gallate,kaempferol-3-o-d-glucopyranoside,quercetin,luteolin,kaempferol from the leaves of the Dimocarpus longan Lour.from different habitats by HPLC.4 To establish fingerprint in ethyl acetate fraction of the leaf of Dimocarpus longan Lour.collected from different habitats by HPLC.Results: 1 Eleven compounds were isolated and identified from petroleum ether extracts and ethyl acetate extracts from the leaf of Dimocarpus longan Lour.as 1-octacosanol(1),lupeol(2),octacosanic acid(3),triacontanoic acid(4),?-sitosterol(5),daucosterol(6),ethyl gallate(7),kaempferol(8),luteolin(9),quercetin(10),kaempferol-3-o-d-glucopyrano side(11).2 The extractive of the ethyl acetate fraction and n-butanol extract of the leaves of Dimocarpus longan Lour.had a high inhibitory activity,to evaluate the activity,which the ?-glucosidase inhibitory activity of ethylgallate,kaempferol,luteolin,quercetin,kaempferol-3-o-d-glucopyrano side in ethyl acetate fraction and lupeol in petroleum ether fraction from the leaf of Dimocarpus longan Lour,who had better inhibitory activity than acarbose.3 The linear calibration rage is 1.624?5.684?g for ethylgallate?0.048?0.168?g for kaempferol-3-o-d-glucopyranoside? 1.624?5.684?g for quercetin ?0.048?0.168?g for luteolin ? 0.324?1.134?g for kaempferol,The average recovery were100.52%?100.68%?100.00%?101.48%?100.40%,The relative standard deviations(RSD)were 0.3%?1.2%?0.6%?1.3%?0.8%(n=9).4 twelve common peaks were identified,which used ethylgallate as reference,by similarity analysis,cluster analysis,principal component analysis were used to identify the leaf of Dimocarpus longan Lour.ten batches of the sample.and all the batches of the sample similarity were over 0.90 and were classified into four categories,there were two main composition factors.Conclusion: Study the chemical constituents of petroleum spirit fraction and ethyl scetate fraction of the leaves of Dimocarpus longan Lour.and eleven chemical constituents were identified,they can offer the theory basis for the other extracts of the leaves of Dimocarpus longan Lour.The extractive of the ethyl acetate fraction and n-butanol extract of the leaves of Dimocarpus longan Lour.had a high inhibitory activity,it can offer the basis to separate the chemical constituents of the n-butanol extract of the leaves of Dimocarpus longan Lour.Optimization extraction process by multi-index comprehensive scoring method to measure diferent habitates of the leaves of the Dimocarpus longan Lour.The HPLC fingerprint was a good repeatability,simple,reliable,which can be used for controlling the leaf of Dimocarpus longan Lour.Quality and providing the basis.