Study On Extaction And Biological Activity Of Flavonoids From Elaeagnus Angustifolia Fruit

Elaeagnus angustifolia(Elaeagnus angustifolia L.)belongs to deciduous trees or small trees.As Uighur medieine(called "Jiga Dan")applied in folk for a long time,E.angustifolia is used to treat spleen and stomach weakness,indigestion,enteritis,diarrhea,cough with lung heat disease.Extracted from the fruit of E.angustifolia,E.angustifolia fruit flavonoids is a eompound having a phenolie hydroxyl group of two benzene rings eonneeted to each other through the central three carbon atoms.Although they are not recognized as vitamin,it is believed that they have nutritional functions in the reaction of organism,known as"vitamin P,,which have antioxidant,antibacterial,antiviral,antitumor,anti-inflammatory,analgesic,anti arrhythmia effect and hepatoprotective activity,and etc.But at the moment,there is few studies of extraction or purification technology and antioxidant or antibacterial mechanism of E.angustifolia fruit flavonoids.In order to exploit the resource better,the thesis mainly focuses on the extraction and purification process and antioxidant and antibacterial effects of total E.angustifolia fruit flavonoids.First of all,two methods,the water bath and homogenate,are employed to extract the flavonoids from E.angustifolia By optimal comparison,the optimal method is chosen to extract Flavonoids from E.angustifolia The results go like this.The optimum parameters of flavonoid extraction from E.angustifolia by the water bath extraction are as follows:The ethanol concentration was 70%,water bath temperature was 85?,material to liquid ratio was 1:30 g·mL-1,extraction time was 30 min,extraction times was twice.The optimum parameters of flavonoid extraction from E.angustifolia by homogenate extraction are as follows:The ethanol concentration was 55%,material to liquid ratio was 1:30 g-mL-1,extraction time was 5 min,extraction times was twice.The maximum yield of extraction of E.angustifolia fruit flavonoids with the water bath method was 4.50%while the homogenate method was 6.57%,By comparing,the homogenate extraction method is more suitable for the extraction of E.angustifolia fruit flavonoids.Second,macroporous adsorption resin was selected in separation and purification of E.angustifolia fruit flavonoids.9 different types of macroporous adsorption resin were screened,including different polarity,the average pore diameter,specific surface area,and 3 kinds of macroporous adsorption resin were selected for further static kinetic curve.Finally,the type AB-8 macroporous adsorption resin was selected for dynamic experiment.The results showed that the optimal process for the separation and purification of E.angustifolia fruit flavonoids were as follows:adsorption rate was 2 BV·h-1,concentration of adsorpting solution was 2.43 mg·mL-1,ethanol concentration was 70%,elution rate was 2 BV·h-1.According to the optimum conditions of purification,the total content of flavonoids of E.angustifolia fruit increased from 9.73%to 49.52%,and the purity increased by 5.09 times,recovery rate was 72%.Hyperoside content increased from 0.53%to 3.07%,and the purity increased 5.79 times.It is further proved that type AB-8 macroporous adsorption resin can be used in separation and purification of E.angustifolia flavonoids.Finally,the antioxidant in vitro and antibacterial experiments that purification on E.angustifolia fruit flavonoids before and after were done.In terms of DPPH,anti hydroxyl radical,anti superoxide anion free radical and reducing power,the experiment showed that the antioxidant order was Vc>pxurified samples of E.angustifolia>E.angustifolia samples before purification.And the ability of DPPH free radical scavenging and the ability to resist the hydroxyl free radicals,the antioxidant capacity of E.angustifolia samples after purification was about two times than before.Furthermore,the ability of anti superoxide anion free radical and the ability to resist iron reduction,the antioxidant capacity of the purified samples of E.angustifolia was much higher than that of the sample before purifieation of E.angustifolia In summary,it ean be seen that the sample after purifieation of E.angustifolia had stronger antioxidant ability.Through the bacteriostatic experiment,conclusions can be drawn.First,the whole effect of the sample after purification from E.angustifolia is better than the samples before purification from E.angustifolia;Second,the E.angustifolia samples,no matter before or after,bacteriostatic effect of three kinds of bacteria is superior to the bacteriostatic effect of three kinds of fungi;Third,in the three kinds of bacteria of E.angustifolia samples,no matter before or after purification,inhibitory effect on Gram negative bacteria is better than gram positive bacteria.And in gram-negative bacteria,the inhibitory effect on Bacillus strain is superior to that bacteria which has Bacillus strain.This artiele selects the homogenate method to extract the E.angustifolia flavonoids,conveniently,high extraction rate and no pollution.Effect of type AB-8 macroporous adsorption resin separation and purification narrow-leaved oleaster flavonoids is remarkable.The combination of these two methods lays a foundation and as a reference for the industrial production of Flavonoids from E.angustifolia the sample of E.angustifolia after purifieation can improve antioxidant ability.And if further studied it can be used in natural cosmetics.The sample of E.angustifolia after purification has certain bacteriostasis to staphylococcus aureus,Bacillus subtilis and Escherichia coli so it can be applied to the development of natural antibacterial.Evidence is provided for the further study of E.angustifolia.