Effect Of Grgdsp Peptide Coating On Pure Titanium Surface On Osteointegration

Purpose In micro-arc oxidation(MAO)of titanium alloy was implanted,then GRGDSP polypeptide was loaded by coupling of dopamine(PDA)to form a composite active coating,in order to enhance esteointegration.Methods 18 adult New Zealand white rabbits were selected to establish a unilateral mandibular model.Ti-MAO(group A),Ti-MAO-PDA(group B)and Ti-MAO-PDA-GRGDSP(group C)were implanted into the rabbits?mandible.The mandibular bone on one side was selected at the 2W,4W,8W to harvest sample.The bone allografts were observed under CBCT.The interface of sample at 4W was observed under scanning electron microscopy(SEM),laser scanning confocal microscope was went to observe the degree of osteoblast activity,torsion test was went to observe osseointegration.Date were compared between groups with one-way ANOVA using SPSS19.0 software package.Results For osseointegration at contact region,group C was better than group A and group B,and group B was better than group A on CBCT,while SEM showed that group C had more new bone aggregated in the contact area between implant and bone;Laser confocal microscopy showed that the fluorescence intensity of group C was significantly higher than that of group B at the junction,especially in group C.The torsional force of group B and C at 8W was 2 times of that of group A,and group C was 8N.cm more than group B was.The difference was statistically significant between group C and group B,comparing to group A(P <0.05).Conclusions Ti-MAO-PDA and Ti-MAO-PDA-GRGDSP coatings can promote osseointegration,of which the Ti-MAO-PDA-GRGDSP coating has the strongest osseointegration effect.