Receptive Change In Heterogeneous Embryo Transplantation Mice

The normal occurrence of embryo implantation is a necessary prerequisite for pregnancy,and embryo implantation or not,not only determined by the uterus,the embryo in the implant process also played a role.Embryo implantation is the result of the interaction between the embryo and the uterus,which requires both normal developmental embryos with implantable ability and a normal uterus with acceptable ability.There is some interaction between the embryo and the uterus,and in the mutual influence,the adjustment is made until the conditions required for embryo implantation are prepared.At present,in the embryo implantation study,the impact of the embryo for the uterus rarely mentioned.We have adopted a method of heterologous embryo transplantation,the development of normal and the same stage of the rat blastocysts and mouse blastocysts are used in the experiment.Blastocysts were transplanted to the uterus of pseudo-pregnant mice to detect the impact of heterologous embryos for the uterus.Real-time fluorescence quantification,in situ hybridization and immunohistochemistry was used to detect the difference of different levels and different molecules.Through the experiment,it was found that blastocysts of different origin had many effects on mouse uterus: firstly,there were differences in the expression of landmark molecules in uterine window.p-STAT3 and Muc1 were significantly attenuated in the uterine cavity epithelium and glandular epithelium under the influence of heterologous rat blastocysts.The expression of ERα in mouse glandular epithelium and matrix was significantly enhanced under the influence of rat blastocyst.Secondly,cell cycle-related molecules,such as Ccnb2,Cyp26b1 the like,gene expression in rat blastocyst transfer group significantly increased,while,Ki67 mRNA levels are significantly upregulated.Finally,the expression of Olr1 and CD55 in the complement system of innate immunity was also different.Olr1 was down-regulated in the BeWo spheroid transplantation group compared with that in the mouse blastocyst transplantation group,while the CD55 was enhanced at the protein level.In addition,in situ hybridization experiments showed that Rpl26 as a regulatory factor for p53 protein levels in the heterogeneous group,the signal range and signal intensity compared to the mouse blastocyst group,have significantly enhanced.In summary,by heterogeneous embryo transfer method,a number of multi-level differences were detected.These changes suggest that the embryo is not only interacting with the uterus during implantation,but also affects the functional changes of the uterus in many ways.At the same time,the uterus also regulates the effects from the embryo through its own regulation,resulting in adaptive responses.