Three Methods To Establish A Rabbit Model Of Traumatic Proliferative Vitreoretinopathy

Objective:To compare the effects of three methods on the rabbit model of traumatic proliferative vitreoretinopathy.Methods:Twenty-four pigment rabbits,48 eyes in total,were randomly divided into four groups of 12 eyes each on the basis of all causing rabbit eye trauma,three experimental groups:Group A:0.3 m L platelet-rich plasma（PRP）,Group B:0.3 m L transforming growth factorβ2（TGF-β₂）（50 ng/m L）,Group C:0.3 m L retinal pigment epithelium（RPE）（1.5×10 ⁶/m L）,control group:Group D:0.3m L saline.Postoperatively,the proliferation of vitreous and retina was observed weekly by fundus photography and ultrasound;the expression ofα-smooth muscle actin（α-SMA）during intravitreal retinal proliferation was detected by western blot（WB）at the fourth week postoperatively and the retinal tissue structure of each group was observed under a microscope by frozen section.Results:At the fourth week after surgery,the mean grades of traumatic proliferative vitreoretinal lesions in the control group and the three experimental groups were 0,3.67,2.33,and 2.50,respectively;the grades of traumatic proliferative vitreoretinal lesions groups were significantly higher in the three experimental groups than those in the control group（P<0.05）,and the grades of traumatic proliferative vitreoretinal lesions in group A were higher than those in groups B and C（P<0.05）The grade of traumatic traumatic proliferative vitreoretinal lesions in groups B and C was not statistically significant（P>0.05）;at the fourth week after surgery,α-SMA expression in the vitreous retina were higher in the experimental groups than in the control group（P<0.05）;at the fourth week after surgery,the sections of the experimental group showed a higher degree of retinal proliferation than that of the control group.Conclusion:On the basis of causing penetrating eye injuries,intravitreal luminal injections of RPE,PRP,and TGF-β₂ all caused traumatic proliferative vitreoretinopathy in rabbits,with vitreous injections of PRP modeling the most pronounced effect.