Study Of The Artemisia Rupestris Extract Effecting In Airway Hyperresponsiveness Caused By Gastroesophageal Reflux

Objective:To observe the effect of Artemisia rupestris on rats with gastroesophageal reflux,and to elucidate the mechanism of treating airway hyperresponsiveness caused by gastroesophageal reflux,providing theoretical basis for its development and utilization.Method:?1?Gastrointestinal peristalsis in mice:50 healthy male Kunming mice,randomly divided into blank group(water 15ml·kg^-1·d^-1),the low(1.5g·kg^-1·d^-1),middle(3g·kg^-1·d^-1),high dose group(6g·kg^-1·d^-1)of Artemisia rupestris extract,and domperidone group(5.0mg·kg^-1·d^-1),10 mice each group.Intragastric administrate them once a day.After the 5th day fasting for 24 hours,but water is allowed..On the 6th day,30 minutes later the administration,intragastric administering them with dextran blue-2000,0.3 ml each,measuring residual rate of gastric pigments in the stomachand intestinal propulsive ratio.?2?Pharmacological intervention:divide the 70 SD rats randomly into 7 groups:sham operation,model group,low,medium,high of Artemisia rupestris,TRPV1 antagonist pepper group and 5HT4R antagonist group.Open the sham operation group along the midline of abdominal wall,exposing the abdominal organs for15 minutes.Then,suture and close the abdominal.The other rats were established with pylorus semiligation,and cardiomyotomy with internal injury.Give the rats postoperative support such as cephalosporin,5%glucose and warm-keeping.After 7 days of modeling,the low(1g·kg^-1·d^-1),medium(2g·kg^-1·d^-1),high(4g·kg^-1·d^-1)doses of Artemisia rupestriswere intragastric administrate,TRPV1 antagonist(0.5?mol·kg^-1·d^-1)and 5HT4R antagonist(1?mol·kg^-1·d^-1)were also abdominal cavity administered for them for 14 days.Measure Airway Resistance and Lung Compliance of rats by Pulmonary function stimulation test.Detecte and analyze the related proteins?TRPV1,5HT4R,SERT?,detecting neurotransmitters?NKA,NKB,SP?and genes?TNF-?,IL-1?,IL-6,LPCAT2,TRPV1,5HT4R and SERT?.Results:?1?Compared with the control group,the Artemisia scopariae group could effectively promote gastric emptying of mice,and the high dose was similar to the domperidone.?2?After modeling,compared with the sham operation group,the rats'weight wassignificantly decreased?P<0.05?.After 14 days of administration,the Artemisia scopariae groups'weight increase was significantly higher than that of the model group?P<0.05?;After excitation of different concentrations of Mch.the percentage of Artemisia scoparia groups'airway resistance increase and compliance was lower than those of model group?P<0.05?.Compared with the sham operation group,the number of neutrophils and monocytes in the model group was significantly higher?P<0.05?.Compared with the model group,the white blood cells of the high and middle dose groups were significantly lower?P<0.05?.?3?ELISA test:In the SP content determination,the model group was significantly lower?P<0.05?when compared with sham operation;the TRPV1 antagonist group and the high-dose group were significantly increased when compared with the model group?P<0.05?.In the determination of NKA and NKB,the model group was significantly higher than that of sham operation?P<0.05?,and the TRPV1 antagonist group,medium and high dose groups were lower than the model group?P<0.05?.In the determination of 5-HT,the model group,the TRPV1antagonist group,the 5HT4R antagonist group,and the low-dose group were significantly lower than the sham operation?P<0.05?.Compared with the model group,the high-dose group was significantly increased?P<0.05?.?4?RT-PCR test:When in the lungs,the expression of TNF-?,IL-1?,IL-6 and LPCAT2 in the model group was significantly higher than that in the sham operation?P<0.05?,the expression of TNF-?in TRPV1antagonist and high dose groups was significantly decreased compared with the model group?P<0.05?,the expression of IL-6 and LPCAT2 in the high-dose group was significantly decreased?P<0.05?.In the esophagus,the expression of TRPV1,TNF-?,IL-1?,IL-6 and LPCAT2 in the model group was significantly higher than those in the sham operation group?P<0.05?,and the expression of 5HT4R and SERT in the model group was significantly decreased?P<0.05?.5HT4R was significantly increased in the TRPV1 antagonist group compared with the model group?P<0.05?,and TNF-?was significantly decreased?P<0.05?.In the esophagus,compared with the model group,the expression of TRPV1,TNF-?,IL-6 and LPCAT2 in the high dose group was significantly decreased?P<0.05?,and the expression of 5HT4R and SERT was significantly increased?P<0.05?.?5?Western blot analysis:Compared with the sham operation group,the expression of TRPV1 in the model group was significantly increased?P<0.05?,and the expression of 5HT4R and SERT was significantly decreased?P<0.05?.Compared with the model group,the high dose group TRPV1 The expression was significantly decreased?P<0.05?,and the expression of 5HT4R and SERT was significantly increased?P<0.05?.Conclusion:Artemisia rupestris can effectively promote gastrointestinal function,improve rats'airway hyperresponsiveness caused by gastroesophageal reflux,and reduce their inflammation index.5-HT vagal pathway may be the main way for Artemisia annua to improve airway hyperresponsiveness induced by gastroesophageal reflux.