| Planting economic crops in the beach area resistant to seawater and salinity is one of the effective measures to reasonably develop and utilize beach.Jerusalem artichoke(Helianthus tuberosus L.)is resistant to salt water and has strong stress resistance.Jerusalem artichoke is also rich in active ingredients that can regulate the physiological function of human body.Jerusalem artichoke has many physiological functions.The paper researches on Jerusalem artichoke inulin.In order to provide theoretical support for exploring the functional mechanism of inulin from Jerusalem artichoke,the paper systematically studies on the alleviated effects of Helianthus tuberosus L.on mice with hyperlipidemia,hyperglycemia and obesity through animal experiments.The main research contents and results are as follows:1.The model mice of hyperlipidemia was established by feeding high fat diet for a long time.The model mice were treated with 9.0‰ saline(model group),3 doses of inulin by low(5g/(kg·d)),mide(10g/(kg·d))and high(20g/(kg·d))(low dose inulin group,middle dose inulin group and high dose inulin group)and drug of Simvastatin Tablets(positive drug group).At the same time,the mice fed with normal diet and 9.O‰ saline were used as blank control and the processing time was 28 days.The effect of Jerusalem artichoke inulin on hyperlipidemia mice were carried out to measure the serum lipid index(total cholesterol(T-CHO),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),body weight,relative peritoneal fat,relative liver weight,hepatic lipids(total cholesterol(T-CHO),triglycerides(TG),antioxidant capacity of liver and intestinal flora,and observe the liver oil red O staining slice.The results are as follows:After the treatment of Jerusalem artichoke inulin,serum and liver lipids were significantly lower;the body weight,relative liver weight and relative fat weight decreased;the content of total superoxide dismutase(T-SOD)in the liver increased significantly,while the malondialdehyde(MDA)content decreased significantly;the results of liver oil red O staining showed that Jerusalem artichoke inulin could reduce the distribution of lipid droplets in the liver,and improve the vacuolar degeneration of liver tissue.There were significant differences in the abundance and diversity of the intestinal flora in hyperlipidemia mice,and the proportion of Bifidobacterium in the intestinal tract was significantly increased or changed after treatment.The results showed that inulin could relieve hyperlipidemia in mice by reducing serum and liver lipid,improving the antioxidant ability of mice with hyperlipidemia,remissing of hepatic steatosis caused by hyperlipidemia and increasing the number of intestinal bacteria.2.The model mice of hyperglycemia was inducted of type 2 diabetes mellitus in mice by intraperitoneal injection of high fat diet combined with intraperitoneal injection of low dose streptozotocin(streptozo tocin,STZ).The model mice were treated with 9.0‰ saline(model group),3 doses of inulin by low(5g/(kg·d)),mide(10g/(kg·d))and high(20g/(kg·d))(low dose inulin group,middle dose inulin group and high dose inulin group)and drug of Metformin Hydrochloride Tablets(positive drug group).At the same time,the mice fed with normal diet,9.0‰ saline were used as blank control and the processing time was 28 days.The effect of Jerusalem artichoke inulin on hyperglycemia mice were carried out to studied through determining the fasting blood glucose(FBG),serum lipid index(total cholesterol(T-CHO),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),body weight,the daily drinking water,relative liver weight,liver related gene expression and intestinal flora.The results are as follows:After the treatment of Jerusalem artichoke inulin,the fasting blood glucose(FBG)was significantly reduced;relative liver weight and average daily drinking amount of mice significantly decreased;serum total cholesterol(T-CHO),triglyceride(TG)and high density lipoprotein cholesterol(HDL-C)levels were significantly reduced;there were significant differences in the abundance and diversity of the intestinal flora in hyperglycemia mice,after treatment the proportion of Bacteroides in the intestinal tract was significantly increased,while the proportion of Lactobacillus group was significantly reduced.The results showed that inulin could relieve hyperglycemia in mice by reducing the high blood glucose fasting blood glucose(FBG),alleviating the hyperglycemia caused by the abnormal increase of serum lipid index,improving the expression of liver lipid genes and increasing the intestinal bacteria quantity.3.In order to investigate the mitigation effect of inulin on obese mice,the obesity mice model was established using high-fat feed.The model mice were treated with 9.0‰saline(model group),2 doses of inulin by low(5g/(kg·d))and high(10g/(kg·d))(low dose inulin group and high dose inulin group)and drug of Orlistat(Orlistat group).At the same time,the mice fed with normal diet,9.0‰ saline were used as blank control and the processing time was 28 days.The effect of Jerusalem artichoke inulin on obesity mice were carried out to measure the body weight,relative liver weight,serum indexes(total cholesterol(T-CHO),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C)and observing the liver oil red O staining slice.The results are as follows:Compared with model group,inulin can reduce mice weight(lowered 9.03%and 7.77%),hepatopancreas index(lowered 13.81%and 44.20%),total cholesterol(lowered 7.91%and 12.02%),HDL-C/LDL-C(lowered 16.39%and 73.51%),staining showed the number of fat droplets in mice liver was less than model group.The results showed that inulin could relieve obesity in mice by decreasing the total cholesterol content in serum,increasing the ratio between high and low density lipoprotein cholesterol,reducing the accumulation of liver fat and reducing relative liver weight.The results showed that inulin could alleviate the hyperlipidemia,hyperglycemia and obesity in mice. |
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