| Objective To investigate the effects of long-chain non-coding RNA-HOTAIR on the malignant biological behaviors of glioma U251 cells and related mechanisms,in order to further clarify the occurrence and development of gliomas and open up new road to treat gliomas.Methods U251 human glioma cells were cultured in logarithmic growth phase and further grouped into control group,negative control group(sh-NC)and experimental group(sh-HOTAIR),followed by MTT colorimetric assay to detect cells proliferation;Detection of HOTAIR mRNA expression level by qRT-PCR;Detection of apoptosis using phosphatidylserine-binding protein-Annexin V-FITC/PI dual-label flow cytometry.The invasiveness of the cells was detected by Transwell chamber in vitro invasion assay;Western Blot was used to detect expression level of apoptosis-related protein Bcl-2,invasion-associated protein MMP-2,PI3K/Akt signaling pathway important proteins protein kinase B(Akt),phosphorylated protein kinase B(P-Akt).Results The results of MTT suggested that the proliferation rate of shRNA-HOTAIR transfected with human U251 glioma cells was lower than that of the control group(P<0.05),and the expression of HOTAIR mRNA after down-regulation of cells transfected with shRNA-HOTAIR by qRT-PCR was significantly lower than that of the control group(P<0.05)Flow cytometry data showed that shRNA-HOTAIR could reduce the apoptosis rate(P<0.05).Western Blot results suggested that shRNA-HOTAIR could reduce the expression of P-Akt,Bcl-2,MMP-2 and increase the expression of Bax.Conclusions Silencing LncRNA-HOTAIR is involved in inhibit cell invasion,promote apoptosis,and reduce cell invasion.The molecular mechanism may be related to activation of PI3K/AKT signal transduction pathway. |
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