Study On The Effect Of 2.5% Sodium Bicarbonate In Sterilizing Toothbrush

Objective:Toothbrushes are a common tool for removing plaque bacteria and oral debris,and play an important role in personal oral hygiene and plaque control,and are essential for maintaining daily oral hygiene.As early as 1920,Cobb proposed the concept that the toothbrush was contaminated after use.Cobb believed that toothbrush contamination was the cause of repeated infections in the mouth.Microorganisms can enter the toothbrush from the oral or external environment,such as contaminated fingers,aerosols from toilet flushes,and bacteria found in wet and humid conditions found in bathrooms.Microorganisms can remain active on the bristles of the toothbrush for a period of time ranging from 24 hours to 7 days.Microorganisms that attach,accumulate and survive on the toothbrush may be transmitted to the individual,which in turn may cause disease.Contaminated toothbrushes may play an important role in many oral and systemic diseases,including sepsis and gastrointestinal,cardiovascular,respiratory and renal diseases.Reducing toothbrush contamination is an effective way to avoid infection.Therefore,the toothbrush should be replaced and disinfected regularly.In this study,2.5% sodium bicarbonate solution and boiling water disinfection toothbrush were used to investigate the effect of 2.5% sodium bicarbonate solution disinfecting toothbrush,and the disinfection effect and optimal disinfection time of 2.5% sodium bicarbonate solution were obtained,which provided suggestions for disinfecting toothbrushes.Especially for patients with respiratory system,gastrointestinal tract,low immunity,etc.,to maintain oral health and avoid infection by disinfecting the toothbrush.Methods:During the period from July to December 2018,college students living in the Seventh House of Xinmin Campus of Jilin University will be studied.A total of 420 toothbrushes were distributed and 420 were recovered.The toothbrush are used for 1~7 days in sequence.For each group of 20 toothbrushes,the experiment was divided into two phases.The first phase used to detect the number of bacteria in the toothbrush using 1~7 days.The second phase used a toothbrush with 1~7 days to use 2.5% sodium bicarbonate solution and Disinfect the boiling water and observe the disinfection effect.Group A(blank control group): rinse under the tap for 20s;Group B(2.5% sodium bicarbonate solution group): soaked in 2.5% sodium bicarbonate solution,the amount of solution is all immersed in the toothbrush head,soaking time is 20 min,rinse for about 20 s after soaking;Group C(boiling water group): Soaked in boiling water above 82 °C,the amount of solution is all immersed in the toothbrush head,soaking time is 5min,rinse for about 20 s after soaking.Evaluation index: number of bacterial coloniesResults:1.Unused new toothbrush does not detect bacteria2.The number of bacterial colonies(CFU/ml)in group A(blank control group)on the 1st to 7th day were 550.10±180.049,1120.40±260.670,1952.30±811.287,2616.00±983.708,5874.00±1985.709,11306.50±4122.485,27356.50±10670.392,respectively.In the first to fourth,the number of bacterial colonies on the toothbrush is relatively small,in a gentle state,and the growth rate is relatively slow;on the 4th to 6th days,the number of bacterial colonies on the toothbrush is relatively large,compared with the first four days.The growth rate is relatively fast;on the 6th to 7th day,the bacteria on the toothbrush increased significantly,and the growth rate was also in a state of rapid growth.3.There was a statistically significant difference(P<0.05)in the number of bacterial colonies(CFU/ml)in group A(blank control group)on days 1~7.4.The number of bacterial colonies(CFU/ml)in group B(2.5% sodium bicarbonate solution group)on day 1~7 was significantly different from that in group A(P<0.05).5.The number of bacterial colonies(CFU/ml)in group C(boiling water group)on the 1st to 7th day was significantly different from that in group A(P<0.05).6.There were no significant differences in bacterial colonies(CFU/ml)between groups B and C on days 1 to 4(P>0.05).7.The number of bacterial colonies(CFU/ml)in group B and group C were significantly different on the 5th to 7th day(P<0.05).Conclusions:1.The new toothbrush that is not used is not contaminated with bacteria.2.The toothbrush is contaminated with a toothbrush for 1~7 days.As the number of days of use increases,the number of bacterial colonies on the toothbrush increases.3.Soak the toothbrush with 2.5% sodium bicarbonate solution to reduce bacteria on the toothbrush.4.Soak the toothbrush with boiling water to reduce the bacteria on the toothbrush.5.Using 2.5% sodium bicarbonate solution compared to boiling water,the 2.5% sodium bicarbonate solution disinfected the toothbrush better from day 5 onwards.6.The number of bacterial colonies on the toothbrush used for 1~4 days is small,and the growth rate of bacteria is slower;the number of bacterial colonies on the toothbrush using 4~7 is higher,especially on days 6~7,bacteria A large increase,and rapid growth.Therefore,it is recommended that the toothbrush be disinfected once after 4 days to keep the toothbrush clean and dry,thus maintaining good oral hygiene.