Differential Expression Of Serum MiRNA In Patients With Damp-heat Constitution Of Primary IgA Nephropathy

ObjectiveUsing genomics theory and methods to identify miRNA-specific expression in serum of patients with primary IgA nephropathy and damp-heat healthy people.Exploring the molecular biomarkers for the diagnosis and evaluation of IgA nephropathy and damp-heat and the effects of the development and development of damp-heat and IgA nephropathy.Providing the basis of molecular biology for disease prevention and treatment,and implementing the concept of disease prevention for treating Chinese medicine.MethodsSubjects were enrolled in the Department of Nephrology,Guangdong Provincial Hospital of Traditional Chinese Medicine,from March 20 to June 2018.The subjects were selected from 10 patients with primary IgA nephropathy and damp-heat quality according to the inclusion and exclusion criteria.There were 10 cases of damp-heat mass and 10 cases of healthy and healthy people.There was no special clinical examination.The final enrolled subjects took 6 ml of venous blood and extracted whole blood RNA using HiPure Universal RNA Mini Kit from Guangzhou Meike Biological Technology and High-throughput sequencing of RNA by Guangzhou Ruibo Biological Technology.With | log2(Fold Change)|?1 as the threshold and P<0.01 as the high significant difference,differentially expressed miRNAs were selected for kegg biochannel enrichment analysis and GO analysis.ResultsA total of 16 miRNAs with significant differences in primary IgA neph ropathy with damp-heat constitution were statistically analyzed,of which 10 were up-regulated and 6 were down-regulated;5 were miRNAs with signi ficant differences in damp-heat constitution,1 of which For up-regulatio n,4 miRNAs were down-regulated.A total of 35 signal transduction and disease pathways were found in the primary IgA nephropathy damp-heat cons titution group compared with the background,which were related to immuni ty and inflammation(including hepatitis and intestine).Road infection,herpes simplex infection,etc.),metabolism,cell cycle,tumor,diabetes,renin-angiotensin-aldosterone system,etc.There are 25 statistically sig nificant signal transduction and disease pathways in the damp-heat health y group,with immunity,tuberculosis and HTLV-I infection,platelet activ ation,endocrine resistance,insulin resistance,prostate cancer,colorec tal cancer,Small cell lung cancer,renin-angiotensin-aldosterone system are closely related.The GO annotation of the candidate target gene in the damp-heat healthy human group is roughly the same in the cell composi tion,molecular function,and biological process as the primary IgA nephr opathy damp-heat patient group,except that the enrichment order is not the same.ConclusionCompared with the healthy control group,there are many differentially expressed miRNAs in the serum of patients with primary IgA nephropathy and damp-heat healthy people.These differentially expressed miRNAs provide a molecular biological basis for disease prevention and treatment.with| log2(Fold Change)|?1 and P<0.05 as general differences.,hsa-miR-548ar-3p and hsa-miR-144-5p were observed in both damp-heat healthy and IgA nephrotic damp-heat mass groups down-regulated.In terms of KEGG enrichment pathway,we found that 13 KEGG pathways in the damp-heat healthy population were consistent with the primary IgA nephropathy damp-heat mass group,indicating that there is a relationship between IgA nephropathy and damp-heat physique.Providing molecular biology basis for the theory of "body disease related"in TCM constitutional theory...