Studies On Secondary Metabolites Of Penicillium Sp. DT10 And Penicillium Brevicompactum

Fungus belong to a large branch of the microbial system,and their secondary metabolite are diverse.Naturally derived secondary metabolites not only provide a natural treasure house for human medicine exploration,but also provide new discoveries for further enriching the chemical structure of compounds.Fungus exist in the special ecological environment will have more complex metabolic mechanisms in the process of adapting to the survival process than in the common growth environment.For this reason,the secondary metabolites are worth exploring.We carried out solid-state fermentation of Penicillium sp.DT10 from the soil of wetland soil,and made separation and analysis of ethyl acetate parts.Finally,five new diterpenoid glucoside,two diterpenoid aglycone,two known analogues,four new ES-242 s compounds,one known analogues were obtained.Their structures and absolute configurations were elucidated based on spectroscopic analyses,acid hydrolysis,ECD calculations or comparison and X-ray crystallography.Secondary metabolites research on another fungus called Penicillium brevicompactum was carried out as well.Based on the same separation methods,two known mycophenolic acid analogues and five known steroids were found in this fungus fermentation.We screened some of the isolated new diterpene glycoside compounds to tested seed germination activity,new compounds showed plant growth regulating activities comparable to those reported in the literature;new ES-242 s bioxanthracenes derivatives and steroids and sesquiterpenes were screened for cytotoxic and anti-inflammatory activities.Some compounds exhibited cytotoxic activity and had potential anti-inflammatory activity.