Studies On The Chemical Constituents And Bioactivities Of Penicillium Sp.sb62

Microorganisms is an important source of secondary metabolites with a variety of chemical structures and diverse biological activities.More than 30,000 natural compounds have been isolated from microorganisms since the 1940s,over 10,000 of which are biologically active.One fungus?Penicillium sp.sb62?which was isolated from the soil near the fibrous roots of Schisandra bicolor var.tuberculata growed in Xinning,Hunan Province of China,was cultured on rice medium for 28 days,and then extracted with ethyl acetate.26 furancarboxylic acids and their derivatives?1a,1b,2,3a,3b,4,5a,5b,6a,6b,7a,7b,12-17 were new compounds?,11 furancarboxylic acids dimers?19-29 were new compounds?,6 carcasses and 6 other type compounds were isolated from Penicillium sp.sb62 by variety of modern chromatographic separation techniques.Compounds 1–4 represent the first class of natural products featuring an unprecedented thiophene moiety.Then,their structures were established on the basis of spectroscopic data analysis.The absolute configurations of some new compounds were determined by comparison of the specific rotation values,modified Mosher's method and single-crystal X-ray diffraction analysis.Some compounds were tested for their antibacterial activity:Compounds 1-4showed inhibitory activities against Monilia albican,Escherichia coli and Staphylococcus aureus with MIC values of 51-64?g/mL and IC₅₀0 values of 5-20?g/mL,while compounds 5–9 with MIC values of 1–10?g/mL and IC₅₀0 values of 0.02-2.99?g/mL.Compound 7 displayed the most notable activities against M.albican?MIC 4?g/mL?,E.coli?MIC 1?g/mL?and S.aureu?MIC 5?g/mL?,while the positive drug showed inhibitory activities against M.albican,E.coli and S.aureus with MIC 10?g/mL?15?g/mL?20?g/mL.