Effect Of Artesunate On The Expression Of ICAM-1 And MMP-9 In Vascular Endothelial Cells Under High Glucose Condition

Objective: To investigate the effects of artesunate on the expression of ICAM-1 and MMP-9 in vascular endothelial cells under high glucose conditions by using artesunate(ART)to treat vascular endothelial cells under high glucose conditions.Methods: Human Umbilical Vein Endothelial Cells(HUVEC)were divided into Glucose(G)group,40 mmol/L G+ART(G40+ART)group,Mannitol,(M)control group,and G40+ DMSO(DMSO)control group.The concentration gradient of G group was 5.5mmol / L G(G5.5),25 mmol / L G(G25),40 mmol / L G(G40);The concentration gradient of M control group was 5.5 mmol /L G + 19.5 mmol/L M(M25),5.5 mmol/L G+ 34.5 mmol/L M(M40),G40+ART group was divided into G40+10ug/ml ART(10A),G40+20ug/ ml ART(20A),G40 + 40 ug/ml ART(40A);the quantity of DMSO in the DMSO control group was the same as the volume of DMSO used to dissolve ART in the 40 A group.The expressions of Intercellular adhesion molecule-1(ICAM-1)and Matrix metalloproteinase-9(MMP-9)were detected by Western blot and Immunofluorescence.Result:Observe the effects of high glucose conditions on the expression of ICAM-1 and MMP-9 protein in HUVEC and the effects of ART on the expression of ICAM-1 and MMP-9 protein in HUVEC under high glucose conditions.The cells were treated with the culture medium of G5.5,G25 and G40,and the expression of ICAM-1 and MMP-9 protein was detected by Western blot and Immunofluorescence.The results showed that the expression of ICAM-1 and MMP-9 in G25 group was higher than that in G5.5 group,and the expression of G40 group was higher than that in G25 group(P<0.01).The cells were treated with 10 A,20A and 40 A.The expressions of ICAM-1 and MMP-9 were detected by Western blot and Immunofluorescence.The results showed that the expression of ICAM-1 and MMP-9 was lower in G40+ART than that in G40,in which the 10 A group was lower than the G40 group,the 20 A group was lower than the 10 A group,and the 40 A group was lower than the 20 A group,the difference was statistically significant,(all P<0.01).2.Observe the effect of high osmotic pressure on the expression of ICAM-1 and MMP-9 protein in HUVEC.For the M control group,cells were treated with medium containing 5.5 mmol/L G+ 19.5 mmol/L M(M25),5.5 mmol/L G+ 34.5 mmol/L M(M40).The expression of ICAM-1 and MMP-9 was detected by Western blot and Immunofluorescence.The results showed that the expression of ICAM-1 and MMP-9 protein was higher in G25 group than that in M25 group,and the expression in G40 group was higher than that in M40 group,(all P <0.01).3.To demonstrate the effect of ART on the expression of HUVEC ICAM-1 and MMP-9 protein under high glucose conditions,DMSO control group was established.The quantity of DMSO in the DMSO control group was the same as the volume of DMSO used to dissolve ART in the 40 A group.The expression of ICAM-1 and MMP-9 protein was detected by Western blot and Immunofluorescence.The results showed that the expression of ICAM-1 and MMP-9 in G40+ART group was higher than that in the DMSO control group,(all P <0.01).Conclusions: 1.The expression of ICAM-1 and MMP-9 protein increased under high glucose condition,which was concentration dependent.2.High osmotic pressure had no significant effect on the expression of ICAM-1 and MMP-9 protein in HUVEC.3.ART can inhibit the expression of ICAM-1 and MMP-9 protein in vascular endothelial cells under high glucose conditions in a concentration-dependent manner.4.In this experiment,it was proved that ART can inhibit the expression of ICAM-1 and MMP-9 proteins in vascular endothelial cells under high glucose conditions at the cellular level,laying the foundation for further study on the changes of ICAM-1 and MMP-9 expression in the mechanism of ART inhibiting retinal neovascularization and leakage.