Clinical Study On Differential Expression Of MiRNA In Liver-gallbladder Dampness-heat Syndrome In Patients With Chronic Hepatitis B

ObjectiveThe previous molecular biology study of the research group by high throu ghputfound that the different expression profiles of miRNAs in different syndro mes of chronic hepatitis B(CHB)might be used as a research basis for TCM syndromes and a potential target for drug action.In this study,the differential ly expressed miRNAs(miR-129-2-3p,miR-762,miR-21-5p,miR-22-3p,miR-30 d-5p,miR-1304-3p)specific to liver-gallbladder dampness-heat syndrome of chr onic hepatitis B were screened out,in order to obtain the differentially express ed miRNAs.On this basis,small sample of traditional Chinese medicine interv ention was performed,so as to explore the expression of miRNA and improve ment of TCM symptoms after drug intervention,to explore the nature of CHB liver-gallbladder dampness-heat syndrome from the perspective of miRNA and to prepare for the next step to provides the basis for the study of the biologi cal nature of TCM syndromes.Methods30 cases of patients with liver-gallbladder dampness-heat syndrome of chronic hepatitis B and 10 cases of patients in the healthy control group were included in this study.The expressions of differentially expressed miRNAs in peripheral blood of patients in the two groups were detected by qPCR technique,so as to obtain the differentially expressed miRNAs in liver-gallbladder dampness-heat syndrome of chronic hepatitis B,also the related target genes prediction and enrichment analysis were performed.Nextly,the small sample intervention was performed in patients with chronic hepatitis B liver-gallbladder dampness-heat.On the basis of Western medicine antivirus(Entecavir Capsule),the patients were treated with traditional Chinese medicine Qingrelishi Decoction(Longdanxiegan Decoction)for four weeks.The expressions of differentially expressed miRNAs were detected by qPCR technique.The expressions of differentially expressed miRNAs were obtained after drug intervention.The peripheral blood was collected before and after intervention,and the liver function and HBV-DNA load were measured.The score of TCM syndromes was calculated.The results were analyzed by SPSS22.0 software.Results1.Compared with the healthy control group,the expression of miR-129-2-3p was significantly increased in patients with liver-gallbladder dampness-heatsyndrome of chronic hepatitis B(P<0.05),which was consistent with the previous experimental result of the research group;There were no significant differences in the expressions of miR-762,miR-21-5p,miR-22-3p,miR-30d-5p and miR-1304-3p(P>0.05).2.After small sampledrug intervention,the expression of miRNA-129-2-3p was significantly lower than that before intervention(P<0.05).3.Aftersmall sampledrug intervention,the traditional Chinese medicine of hypochondrium distending pain,reduced appetite,anorexia were significantly alleviated compared with those before intervention(P<0.05).ConclusionsmiR-129-2-3p is a significant differentially expressed miRNA in liver-gallbladder dampness-heat syndromeof chronic hepatitis B.Its functions and regulatory pathways involve TRP inflammation regulatory pathway,chemokine signaling pathway,cell apoptosis and cancer-related pathway,etc,may be closely related to the presence of long-term inflammatory liver damagein in liver-gallbladder dampness-heat syndrome in patients with chronic Hepatitis B.It is preliminarily speculated that miR-129-2-3p is a characteristic miRNA in liver-gallbladder dampness-heat of chronic hepatitis B,which provides a direction for revealing the biological nature of combination of disease and syndrome at the level of post-transcriptional regulation of gene expression.