Upregulated Expression Of ACTL8 Contributes To The Invasion And Metastasis And Indicates Poor Prognosis In Colorectal Cancer

Background: Colorectal cancer(CRC)is one of the most common malignant tumors in humans,and its incidence rate ranks third among men's various cancers and second in women.At present,there is still no effective treatment for high invasive CRC.Therefore,it is important to find markers related to CRC invasion and metastasis for the development of new therapeutic methods.ACTL8 is a member of the CT antigens.There are only few studies on the role of ACTL8 in malignant tumors.The aim of this study is to investigate the expression,eclinical significance and mechanism of ACTL8 protein in colorectal cancer.Materials and methods: Analyze the expression of ACTL8 through GEPIA big data.Human CRC tissues and cell lines,and paired adjacent non-tumor tissues and human intestinal epithelial cell lines were obtained to evaluate the expression of ACTL8.ROC determined the boundary value of high and low expression of ACTL8.The association between protein expression of ACTL8 and clinicopathological parameters and prognosis of CRC patients was examined.The biological functions of ACTL8 in the invasion and metastasis of CRC were determined by wound healing and transwell invasion assays after silencing of ACTL8 in CRC cell lines.The key proteins associated with ACTL8 were predicted using STRING and GCBI databases.Quantitative reverse transcription PCR and Western blotting were used to identify the potential target genes of ACTL8.Results: It was found that ACTL8 was upregulated in human CRC tissues and cell lines.The expression of ACTL8 was positively associated with poor differentiation,invasion and metastasis,postoperative infection,and poor prognosis,but negatively associated with proximal margin length.In addition,silencing of ACTL8 significantly decreased the capacity of invasion and migration in HT29 and SW620 CRC cell lines.Moreover,silencing of ACTL8 significantly decreased the expression of TRIM29 in HT29 and SW620 CRC cell lines.Conclusion: These results suggest that ACTL8 plays a key role in the invasion and metastasis of CRC,and TRIM29 may be involved in the ACTL8-mediated poor prognosis of CRC.