Quality Evaluation Of Platelet-rich Plasma In Vitro Preservation

Objective: In this study,Platelet-rich Plasma(PRP)was collected by autologous blood technology,and the quality of Platelet-rich Plasma was compared with that of Platelet-rich Plasma at the end of extracorporal circulation and after protamine and heparin transfusion.Methods: Selected 20 patients undergoing elective in descending aorta general anesthesia surgery,the American Society of anesthesiologists(American Society of Anesthesiology,ASA)Ⅲ level,aged 35 to 65 years old,BMI18.5-27 kg/ ㎡,preoperative HB > 130 g/L,HCT > 35%,normal coagulation function.All patients completed the anesthesia program with the same anesthesiologist.Vital signs monitoring after home,using the same patients with anesthesia induction,lidocaine sprayed throat after complete tracheal intubation,in central venous puncture catheter after according to the situation appropriate for patients with autologous blood platelet rich plasma separation,through peripheral vein to join 1 g 500 ml of calcium plus or lactic acid rehydration salinger fluid to maintain hemodynamic stability,cycle instability can be used to maintain vascular active drugs.Whole blood was added with sodium citrate(10:1)for anticoagulation.The same anesthesia regimen was used for maintenance during the operation.Through comparison,the method of autologous blood were separately measured after separation(T1)of platelet rich plasma(PRP)and after extracorporeal circulation,protamine neutralization heparin after back to lose(T2),platelet rich plasma(PRP),platelet count(Plt),average blood platelet volume(MPV),platelet distribution width(PDW),plasma PH,thrombosis,elastic graph(TEG)R value and MA,autologous blood separation technology separation platelet rich plasma(PRP)process is finished by extracorporeal circulation.Results: The platelet count(Plt)of prp-rich plasma(T1)was lower than that of the platelet count(Plt)of the platelet rich plasma(T2)after PRP separation,but there was no significant difference(P >0.05).Platelet-rich plasma(T1)showed a significant but not statistically significant decrease in platelet distribution(PDW)compared with that at the time of transfusion(T2)(P>0.05).The mean platelet volume(MPV)of the isolated PRP(T1)was significantly lower than that of the intravenous(T2),but not statistically significant(P>0.05).After separation,the PH of PRP(T1)was lower than that at the time of transfusion(T2),but there was no significant difference(P>0.05).The R value of platelet rich plasma(T1)after separation was higher than that at the time of transfusion(T2),but there was no significant difference(P>0.05).After separation,the MA value of platelet rich plasma(T1)was significantly lower than that at the time of transfusion(T2),but there was no statistical significance(P>0.05).Conclusion:The mass effect of platelet rich plasma(PRP)isolated by autologous blood separation technique is still within the normal range when stored in the moving preservation chamber in vitro.