Effect Of MiR-212 On Biological Function In Bladder Cancer T24 Cells By Targeting HMGA2

Bladder cancer is the most common malignancies of urinary system in our country,it's the 6th most frequently diagnosed cancer worldwide,and the mortality rate ranks 9th in terms of death rate.It mainly occurs in transitional epithelial cells of the bladder and is directly exposed to urine,also known as urothelial cancer.About 70-75% of bladder cancer is classefied of non-muscular invasive bladder cancer(NMIBC),and 25-30% is classefied of muscular invasive(MIBC).The 5-year survival rate for patients with non-muscle invasive bladder cancer are over 90 %.while about 5% of the 25-30% patients with muscular invasive bladder cancer had distant metastasis,and a considerable proportion(25%)of the regional lymph nodes had metastasis.The 5-year survival rate of patients with locally advanced or metastatic lesions is about 15%.The treatment methods include surgery,intravesical chemotherapy,and immunotherapy.Cystoscopy is an effective tool for detecting bladder cancer.However,a series of complications are prone to occur after this examination.In order to better diagnose and follow up the treatment of bladder cancer,more accurate biomarkers need to be developed.Micro RNAs(mi RNAs)are small(17~25nucleotides),non coding RNA that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3' untranslated regions(3'-UTR)of m RNAs.Research shows that the abnormal expression of mi RNAs has been identified in various types of cancer,which is involved in apoptosis,proliferation,migration and invasion.Therefore,to explore the expression of micro RNAs in bladder cancer and its effect on biological function is of great significance for further exploring the pathogenesis of bladder cancer and finding new targets.Objective: To investigate the effect of miR-212 on biological behavior of bladder cancer T24 cells by targeting HMGA2.Methods: QRT-PCR method was used to detect the expression level of mi R-212 and HMGA2 in 31 cases of bladder cancer tissues and adjacent normal tissues.Immunohistochemistry method was used to detect the expression level of HMGA2 in 31 cases of bladder cancer tissues and adjacent normal tissues.Human bladder cancer T24 cells were divided into two groups: mi R-212 group(transfected with mi R-212 mimics)and the control group(transfected with blank mi R-212 NC).After 48 hours of incubation,MTT method and Transwell experiment was used to observe the level of cell proliferation,invasion and migration.wounding heal experiment was used to observe the level of cell migration.Western blot method was used to detect the protein expression level of HMGA2.Luciferase reporter experiments was used to verify whether HMGA2 is the downstream target gene of mi R-212.Results:Compared with normal tissues,the expression of mi R-212 in bladder cancer tissues decreased significantly(P<0.05).the expression of HMGA2 in bladder cancer tissues increased significantly(P<0.05).Compared with the control group,mi R-212 group could inhibit cell proliferation,migration and invasion,and decrease the level of HMGA2 protein expression(P< 0.05).Online database and luciferase reporter experiments predicted and verified that HMGA2 is the downstream target gene of mi R-212.Compared with the control group,mi R-212 group could inhibit cell proliferation,migration and invasion,and decrease the level of HMGA2 protein expression..The online database and luciferase report experiments predicted and verified that HMGA2 was the downstream target gene of mi R-212.Conclusion: miR-212 could change the level of cell proliferation,migration and invasion in bladder cancer by targeting HMGA2,thus affecting the development of bladder cancer.