Mechanism Of Ginseng On Red Blood Cell Function Recovery After Exhausted Exercise And Product Development

Objective:In this paper,by studying the effects of ginseng polysaccharides on the red blood cell structure and functional recovery of rats after exhaustive exercise,based on the"qi and blood theory",the biological mechanism of ginseng"Dabuyuanqi"was preliminarily explained from the perspective of ginseng recovery of red blood cells.Methods:1.A model of H2O2-induced red blood cell oxidation and a rat"exhaustion"model were established.2.Using H₂O₂ as the inducer,and using the hemolysis rate as an index to investigate the induction concentration,and on the basis of determining the optimal induction concentration,establish an in vitro erythrocyte oxidative stress model;based on the theory of"Routine Qi Consumption"in traditional Chinese medicine,adopt a modified rat The model of"exhaustion"in rats was established by weight-bearing swimming.Screening of in vitro ginseng active ingredients and screening of in vivo onset time.3.Screening of in vitro ginseng active ingredients and screening of in vivo onset time H₂O₂ induced erythrocyte oxidative damage model was used to screen out the active ingredients of ginseng;rat exhausted swimming model was established,and the test components were the post-exhaustion administration group and the post-exhaustion self-recovery group.The detection was 0.5h,1h,2h,At 4h,8h,12h,and 24h,the difference between the contents of erythrocyte ROS and serum ATP in the administration group and the self-recovery group was used to screen the onset time of the drug.4.Study on the effects of ginseng polysaccharides on blood indexes and mechanical properties A rat exhaustive swimming model was established,and blood pH,pCO₂,pO₂,cCa²⁺,cGlu and whole blood viscosity were measured in each group,and serum ATP,ATPase,SA and EPO contents in each group were measured.5.Study on the effects of ginseng polysaccharides on the structure and function of red blood cells A rat exhaustive swimming model was established,and the red blood cell hemolysis rate of each group was detected by ultraviolet method;scanning electron microscopy was used to detect the red blood cell morphology;immunofluorescence method was used to detect the distribution and fluorescence intensity of Band 3 protein;Western Blot method was used to detect the expression of Band 3 protein;Antioxidant ability indicators?ROS,SOD,MDA,CAT?,red blood cell membrane proteins(Na⁺-K⁺-ATPase,Ca²⁺-Mg²⁺-ATPase)and key enzymes of glycolytic pathway?PK,HK,PFK?activity,detected by western blot technology Band 3 protein expression.6.Development of ginseng fermentation products The fermentation process was used to examine the three factors of pH,inoculum amount,and fermentation time,and an orthogonal test was used to screen the optimal fermentation process,and the changes in total antioxidant capacity before and after fermentation and the differences in the effects on the hemolysis rate of red blood cells were examined.Results:1.The optimal concentration of H₂O₂-induced erythrocyte oxidative damage model is 40mmol·L^-1,and the rat model of oxidative stress and"exhaustion"in vitro has been successfully established.2.Through screening,it was found that 25mg,50mg,and 100mg of ginseng polysaccharides had the most significant recovery of erythrocyte hemolysis rate,so ginseng polysaccharides were selected for follow-up research;ROS and ATP indexes recovered most significantly after 1 hour of exhaustion.Select 1 h after exhaustion for subsequent test indicators.3.The results showed that compared with the blank group,the pH,cGlu,ATP,ATPase,and SA contents of the model group were reduced,and the indicators were restored after administration and there were significant differences;pCO₂,pO₂,cCa²⁺,EPO,and total The blood viscosity increased significantly.The indexes in the self-recovery group recovered slightly but the recovery was not obvious.After the administration,the indexes became normal,and all of them showed dose dependence.4.Compared with the control group,the hemolysis rate in the exhausted group increased,and the hemolytic rate gradually returned to normal levels after administration;scanning electron microscopy results showed that the red blood cell surface shrank after exhaustion,and the bulge increased.The morphology gradually returned to a round and smooth morphology;immunofluorescence results showed that the red blood cells of the control group were fluorescent green and maintained a double-concave round disc shape.The exhausted group was severely deformed and the fluorescence intensity decreased,indicating that Band 3protein clustered after exhaustion.Cross-linking,red blood cell surface fluorescence intensity returned to normal state after administration;red blood cell antioxidant capacity,membrane protease and key enzyme activities of glycolytic pathway were significantly reduced after exhaustion,and returned to normal state after administration,all were meter-dependent.The orthogonal test was used to select the best fermentation technology for ginseng extract fermentation:fermentation pH 6.5,inoculation amount 4.5%,and fermentation time 24h.The total antioxidant capacity before and after fermentation was?0.4094±0.0157?mM and?1.1683±0.0151?mM,respectively.After fermentation,the total antioxidant capacity is enhanced,and the hemolysis rate of red blood cells under the oxidative stress model is better restored.Conclusion:Based on the"qi and blood theory",ginseng polysaccharides can improve the function of red blood cells by improving the deformability of red blood cells under oxidative stress injury and the expression of the key membrane protein Band 3.A great health product with better antioxidant capacity.