EBV And Hp Infections In Gastric Cancer Tissues And Their Correlation With HER2,P53,Ki67 Gene Expression

Objective:This study is aimed to evaluate the correlation between Epstein-Barr virus,Helicobacter pylori infection and the expression of human epidermal growth factor receptor-2,P53 and ki-67 in Gastric Cancer tissues,which is helpful to determine the correlation between those two pathogen and gastric cancer.Methods:A total of 78 cases of tissue wax blocks for radical gastric cancer surgery in Yueyang Second People's Hospital from December2017 to December 2019 were collected,and the gender,age,degree of differentiation of gastric adenocarcinoma,depth of invasion,presence or absence were collected at the same time.Clinical data such as lymph node metastasis and clinical staging.In situ hybridization was used to detect EBV infection in gastric cancer tissues,and immunohistochemical methods were used to detect the status of HP infection and the expression of HER2,P53,Ki67 and other proteins in gastric cancer tissues.Mann-whitney U test was used to compare the expression of EBV,HP infection and protein expression of HER2,P53,Ki67,etc.in gastric cancer tissues.Chi-square test and Fisher exact probability method were used for counting data.The Kruskal-Wallis H rank sum test was used to compare the expression of P53 and Ki67 proteins between the EBV or HP infection group and the combined infection group.Spearman rank sum test was used to detect the correlation of P53 and Ki67 protein expression in gastric cancer tissues in HP group.Kappa consistency analysis was used to check whether EBV and HP infections were consistent in gastric cancer tissues.Results:(1)Among 78 specimens of gastric cancer patients,the positive rate of EBV infection was 12.8%(10/78)and the positive rate of HP was 57.7%(45/78).Consistent analysis was performed on the results of EBV and HP infection(Kappa=-0.127,P=0.058),there is no consistency between the two infections.(2)There were no significant differences between EBV and HP infections in gastric cancer tissues,including gender,age,lymph node metastasis,gastric adenocarcinoma differentiation,depth of invasion,and clinical stage.(3)The expression of HER2,P53,Ki67 and other proteins in gastric cancer tissues were not significantly different from sex,age,lymph node metastasis,adenocarcinoma differentiation,invasion depth,and clinical stage.(4)The expressions of P53 and Ki67 proteins were significantly different from Hp infection(U=369.500,P=0.00;U=386.000,P=0.000),spearman correlation analysis was performed on the expression of Hp and P53 and Ki67 proteins.There was a positive correlation between the expression of P53(r_s=0.477,P=0.000)and Ki67(r_s=0.415,P=0.000)and Hp infection.There was no significant difference in HER2protein expression between Hp-positive group and Hp-negative group.(5)There was no significant difference in the expression of P53 and Ki67 protein between EBV positive or HP positive group and EBV combined HP infection group(P>0.05).Conclusion:(1)There were significant differences in the expression of P53 and Ki67 in gastric cancer tissues between the HP infection group and the non-infection group,and Hp infection was positively correlated with the expression of P53 and Ki67.Hp infection in gastric cancer tissues may promote the above protein expression.(2)EBV or HP infection and EBV and Hp infection in gastric cancer tissues have no effect on the enhancement of P53 and Ki67 protein expression,respectively,and there is no correlation between them.EBV and Hp may be two independent factors in the development of gastric cancer.