Correlation Analysis Between EBV And Hp Infection In Gastric Carcinoma And The Possible Carcinogens Of Hp Associated Gastric Carcinoma

[Objective]1. To disscuss the relationship between the Epstein-Barr virus (EBV) and Helicobacterpylori (Hp) infection and the gastric carcinoma, further research the interactive processof the EBV and Hp.2. To explore the possible carcinogens between the Hp-related virulence and the gastriccarcinoma.[Methods]1. Adopting the techniques of polymerase chain reaction (PCR) and In situ hybridization(ISH) to investigate the EBV infection results of100cases of gastric carcinoma tissuesand82cases of gastritis tissues.2. Employing the method of PCR to detect the Hp infection results of100cases of gastriccarcinoma tissues and82cases of gastritis tissues.3. Utilizing the method of PCR to examine the expression results of Hp-related virulencegenes (cagA, vacAs, vacAm and iceA1) in Hp-positive gastric carcinoma tissues andgastritis tissues.[Results]1. The ratio of positive EBV infection in gastric carcinoma cases is higher than the gastritiscases (9.0%versus0.0%, P＜0.05). The percentage of EBV infection in gastric cardicand body carcinoma is higher than that in gastric antrum carcinoma (16.3%versus2.0%,P＜0.05). The proportion of EBV infection in poorly differentiated carcinoma is higher than that in well-differentiated carcinoma (15.7%versus2.0%, P＜0.05). Moreover,EBV coded RNA functionality expression can be observed in almost all the core part ofEBV-positive gastric carcinoma tissues. The discrepancy of the ratios of EBV infectionin different gender, age, nationality, neoplasm staging, and with lymph node metastasisor not are no statistical meaning.2. The ratio of positive Hp infection in gastric carcinoma cases is higher than the gastritiscases (56.0%versus40.2%, P＜0.05). The percentage of Hp infection in poorlydifferentiated carcinoma is higher than that in well-differentiated carcinoma (66.7%versus44.9%, P＜0.05). The disparity of the ratios of Hp infection in different gender,age, nationality, blood types, occur positions, neoplasm staging, and with lymph nodemetastasis or not are no statistical meaning.3. The EBV infection is irrespective to the Hp infection in the functional process of gastriccarcinoma (r=0.137, P＞0.05).4. In Hp positive gastric mucosa tissues, the positive percentages of Hp virulence genes ofcagA, vacAs1, vacA m1, vacA m2, and iceA1are84.27%、96.73%,48.31%,51.69%,and86.51%, respectively. The positive percentages of Hp virulence genes of vacAs1/mand vacAs1/m2are47.19%and49.44%, respectively. In the total tissue samples, theexpression of Hp virulence genes of vacAs2and the combination of vacAm1andvacAm2.5. In gastric carcinoma cases and gastritis cases, the positive expression ratios of Hpvirulence genes, cagA (85.71%versus81.82%, P＞0.05), vacAs1/m1(51.79%versus39.39%, P＞0.05), vacAs1/m2(44.64%versus57.58%, P＞0.05), iceA1(91.07%versus78.79%, P＞0.05), the combination type of cagA/vacA and s1m1/iceA1(41.07%versus21.21%, P＞0.05) and the combination type of cagA/vacA and s1m2/iceA1(33.93%versus45.45%, P＞0.05), have no statistical meaning.[Conclusions] 1. The EBV infection is the possible dangerous factor of gastric carcinoma, which normallyinvades the middle and top gastric tissues and relates to the malignant development ofgastric carcinoma probably.2. Hp is another significantly dangerous biology factor that participates in the malignantdevelopment of gastric carcinoma, which is independent of the existence of EBV.3. Despite that cagA, vacA s1, and iceA1are the advantageous Hp expression genes in thisresearch, the expression of the virulence genes are irrespective to the occurrence ofgastric carcinoma. Thus these virulence genes cannot be adopted to predict theoccurrence and development of Hp-related gastric carcinoma.