Detection Of Serum Vitamin A Levels In Premature Infants And Its Clinical Significance

Objectives:Through this study,139 cases of premature infants for determination of serum concentration of vitamin A(VA),to investigate serum VA levels in preterm infants with different fetal sizes,gestational age,birth weight,critical neonatal case score(NCIS),and preterm infants with infectious and non-infectious diseases,provide reference basis for prevention and treatment of premature babies VA lackMethods:A total of 139 premature infants who met the inclusion criteria and were admitted to the department of neonatology of the second affiliated hospital of kunming medical university from January 2019 to January 2020 were selected as subjects.2ml venous blood was extracted within 24 hours after admission and serum VA concentration was determined by HPLC.According to fetal rank,gestational age,birth weight,infectious diseases or non-infectious diseases,and according to the NCIS score,the enrolled premature infants were divided into the non-critical group,the critical group and the extremely critical group,and the serum VA levels were compared.Use Excel to collect and organize data.SPSS 23.0 statistical software was used for statistical analysis.Results:1.here were 139 premature infants,including 63 boys and 76 girls.There were 87 cases of single fetus and 52 cases of twin fetus.The serum VA data showed a skewed distribution,and the overall VA level was[0.15(0.11,0.19)]mg/L.Among them,the VA level of male infants was[0.15(0.12,0.21)]mg/L,and that of female infants was[0.14(0.11,0.18)]mg/L.There was no statistical significance in gender difference of VA level(P>0.05).The VA level of single-born premature infants was[0.16(0.13,0.21)]mg/L,and that of double-born premature infants was[0.12(0.07,0.16)]mg/L.The VA level of single-born preterm infants was higher than that of two-born preterm infants,and the difference was statistically significant(P<0.001).2.139 premature infants had normal serum VA level[(0.31-0.50)]mg/L1(0.7%),with a level of 0.32mg/L.Suspected subclinical deficiency[(0.20-0.30)]mg/L in 29 cases(20.9%),with a level of[0.23(0.21,0.25)]mg/L.Deficiency[(<0.20mg/L)]in 109 cases(78.4%),the level is[0.13(0.10,0.16)]mg/L.There were no cases of VA overdose.The overall VA deficiency rate was 78.4%.3.Serum VA concentrations of premature infants at different gestational ages:there was no statistical significance(P=0.087)between the early premature infant group[0.18(0.16,0.22)]mg/L.the mid-term premature infant group[0.13(0.1 1.0.21)]mg/Land the late premature infant group[0.14(0.11,0.18)].4.Serum VA concentration of premature infants with different birth weight:There were statistically significant differences between the very low birth weight group[0.15(0.11,0.17)]mg/L,the low birth weight group[0.13(0.09.0.17)]mg/L and the normal birth weight group[0.16(0.14,0.22)]mg/L(P=0.013).Further comparison showed that there were statistically significant differences between the low birth weight group and the normal birth weight group(P=0.004).Spearman level correlation analysis(correlation coefficient r=0.253,P=0.004),and the scatter plot fitted by linear regression was as follows:There was a positive linear relationship between serum VA concentration in low birth weight group and normal birth weight group.There was a positive correlation between serum VA concentration and birth weight in premature infants.5.VA concentrations in premature infants with different NCIS scores:There was statistically significant difference between the non-risk group[0.16(0.13,0.21)]mg/L and the critical group[0.13(0.10,0.16)]mg/L(P=0.009).Spearman level correlation analysis(correlation coefficient R=0.222,P=0.009),and the scatter plot fitted by linear regression showed that there was a positive linear influence relationship between different NCIS scores and the data points of serum VA concentration.There was a positive correlation between serum VA concentration and NCIS score in premature infants.6.Effect of infectious diseases on serum VA level of premature infants:the comparison between the infectious diseases group[0.13(0.10,0.16)]mg/L and the non-infectious diseases group[0.16(0.13,0.21)]mg/L was statistically significant(P=0.003).The serum VA level of premature infants in the infectious disease group was significantly lower than that in the non-infectious disease group.Conclusions:1.In this study,the overall VA level of premature infants was[0.15(0.11,0.19)]mg/L.The VA deficiency rate of premature infants was higher,reaching 78.4%.There was no gender difference in seruum VA level in premature infants.The VA level of twin premature infants was lower than that of single premature infants.;The VA nutritional status of premature infants,especially those born in twins,should be further improved;2.When the birth weight of premature infants is ?1.5kg,the serum VA concentration of premature infants is positively correlated with the birth weight,that is,the VA concentration of premature infants increases with the increase of birth weight;3.Serum VA concentration in premature infants is positively correlated with NCIS score,that is,the more sick the premature infants are,the lower their VA level is,and the VA nutritional status of critically ill premature infants should be paid close attention to;4.The serum VA level of premature infants with infectious diseases is significantly lower than that of premature infants without infectious diseases,and infection is one of the risk factors affecting serum VA level.